Literature summary for 1.1.1.37 extracted from

Liszka, A.; Schimpf, R.; Cartuche Zaruma, K.I.; Buhr, A.; Seidel, T.; Walter, S.; Knuesting, J.; Dreyer, A.; Dietz, K.J.; Scheibe, R.; Selinski, J.
Three cytosolic NAD-malate dehydrogenase isoforms of Arabidopsis thaliana on the crossroad between energy fluxes and redox signaling (2020), Biochem. J., 477, 3673-3693 .

Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
C125S	the mutant of isoform cyMDH1 shows slightly increased activity compared to the wild type enzyme. The mutant of isoform cyMDH2 shows slightly decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C131S	the mutant of isoform cyMDH3 shows decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C155S	the mutant of isoform cyMDH1 shows slightly increased activity compared to the wild type enzyme. The mutant of isoform cyMDH2 shows slightly decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C161S	the mutant of isoform cyMDH3 shows decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C252S	the mutant of isoform cyMDH1 shows strongly decreased activity compared to the wild type enzyme. The mutant of isoform cyMDH2 shows slightly decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C258S	the mutant of isoform cyMDH3 shows decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C279S	the mutant of isoform cyMDH3 shows wild type activity	Arabidopsis thaliana
C292S	the mutant of isoform cyMDH1 shows slightly increased activity compared to the wild type enzyme. The mutant of isoform cyMDH2 shows slightly decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C298S	the mutant of isoform cyMDH3 shows wild type activity	Arabidopsis thaliana
C2S	the mutant of isoform cyMDH3 shows strongly increased activity compared to the wild type enzyme	Arabidopsis thaliana
C330S	the mutant of isoform cyMDH1 shows slightly decreased activity compared to the wild type enzyme and is not inhibited by diamide. The mutant of isoform cyMDH2 shows decreased activity compared to the wild type enzyme and is not inhibited by diamide	Arabidopsis thaliana
C336S	the mutant of isoform cyMDH3 shows wild type activity	Arabidopsis thaliana
C79S	the mutant of isoform cyMDH1 shows slightly increased activity compared to the wild type enzyme. The mutant of isoform cyMDH2 shows slightly decreased activity compared to the wild type enzyme	Arabidopsis thaliana
C85S	the mutant of isoform cyMDH3 shows slightly decreased activity compared to the wild type enzyme	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
Diamide	isoforms cy MDH1 and cyMDH2 are reversibly inactivated by diamide treatment. Both NADH and GSH separately or together prevented inactivation of cyMDH1 and cyMDH2 by diamide	Arabidopsis thaliana
additional information	isoform cyMDH3 maintains its low basal activity upon oxidation with 0.5 mM diamide	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Arabidopsis thaliana	5829	-
nucleus	nuclear localization of cyMDH isoforms is significantly increased under oxidizing conditions in isolated Arabidopsis protoplasts, in particular of isoform cyMDH3	Arabidopsis thaliana	5634	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	gel filtration	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-malate + NAD+	Arabidopsis thaliana	-	oxaloacetate + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	ecotype Columbia	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose 6 column chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate + NAD+	-	Arabidopsis thaliana	oxaloacetate + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 35000, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
cyMDH1	isoform	Arabidopsis thaliana
cyMDH2	isoform	Arabidopsis thaliana
cyMDH3	isoform	Arabidopsis thaliana
NAD-malate dehydrogenase	-	Arabidopsis thaliana
NAD-MDH	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)