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Literature summary for 1.1.1.37 extracted from

  • Tomaz, T.; Bagard, M.; Pracharoenwattana, I.; Linden, P.; Lee, C.P.; Carroll, A.J.; Stroeher, E.; Smith, S.M.; Gardestroem, P.; Millar, A.H.
    Mitochondrial malate dehydrogenase lowers leaf respiration and alters photorespiration and plant growth in Arabidopsis (2010), Plant Physiol., 154, 1143-1157.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
gene mmdh1, genotyping Arabidopsis thaliana
gene mmdh2, genotyping Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of knockout single and double mutants, homozygous T-DNA insertion lines for single and double mutations, for the highly expressed mMDH1 and lower expressed mMDH2 isozymes, mmdh1mmdh2 mutant has no detectable mMDH activity but is viable, albeit small and slow growing. Quantitative proteome analysis of mitochondria shows changes in other mitochondrial NAD-linked dehydrogenases, indicating a reorganization of such enzymes in the mitochondrial matrix, phenotypes, overview. Complementation of mmdh1mmdh2 with an mMDH cDNA recovers mMDH activity, suppressed respiratory rate, ameliorated changes to photorespiration, and increases plant growth Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion highly expressed mMDH1 and lower expressed mMDH2 isoforms Arabidopsis thaliana 5739
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Arabidopsis thaliana
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LKA3 mMDH2; isozymes mMDH1 and mMDH2, eight genes encoding isoforms of NAD-dependent MDH
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Arabidopsis thaliana Q9ZP06 mMDH1; isozymes mMDH1 and mMDH2, eight genes encoding isoforms of NAD-dependent MDH
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Source Tissue

Source Tissue Comment Organism Textmining
leaf from long-daygrown plants Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Arabidopsis thaliana oxaloacetate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
mitochondrial malate dehydrogenase
-
 Arabidopsis thaliana
mitochondrial MDH
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 Arabidopsis thaliana
mMDH
-
 Arabidopsis thaliana
NAD-dependent MDH
-
 Arabidopsis thaliana
NAD-MDH
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana
NADH
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
additional information inverse correlation between mMDH and ascorbate content Arabidopsis thaliana
physiological function mMDH has a role in maximizing photorespiratory rate. The slow-growing mmdh1mmdh2 mutant has elevated leaf respiration rate in the dark and light, without loss of photosynthetic capacity, suggesting that mMDH normally uses NADH to reduce oxaloacetate to malate, which is then exported to the cytosol, rather than to drive mitochondrial respiration. Increased respiratory rate in leaves can account in part for the low net CO2 assimilation and slow growth rate of mmdh1mmdh2. Loss of mMDH also affects photorespiration with alterations in CO2 assimilation/intercellular CO2 at low CO2, and the light-dependent elevated concentration of photorespiratory metabolites Arabidopsis thaliana
physiological function mMDH has a role in maximizing the photorespiratory rate. The slow-growing mmdh1mmdh2 mutant has elevated leaf respiration rate in the dark and light, without loss of photosynthetic capacity, suggesting that mMDH normally uses NADH to reduce oxaloacetate to malate, which is then exported to the cytosol, rather than to drive mitochondrial respiration. Increased respiratory rate in leaves can account in part for the low net CO2 assimilation and slow growth rate of mmdh1mmdh2. Loss of mMDH also affects photorespiration with alterations in CO2 assimilation/intercellular CO2 at low CO2, and the light-dependent elevated concentration of photorespiratory metabolites Arabidopsis thaliana