Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21pLys cells | Phytophthora infestans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxaloacetate | - |
Phytophthora infestans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
oxaloacetate | recombinant wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.026 | - |
NADH | recombinant wild type cytosolic MDH, at 25°C | Phytophthora infestans | |
0.035 | - |
oxaloacetate | native wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.086 | - |
NADH | recombinant wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.176 | - |
oxaloacetate | recombinant wild type cytosolic MDH, at 25°C | Phytophthora infestans | |
0.21 | - |
NAD+ | native wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.21 | - |
NADH | native wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.21 | - |
NAD+ | recombinant wild type cytosolic MDH, at 25°C | Phytophthora infestans | |
0.37 | - |
NAD+ | recombinant wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.64 | - |
(S)-malate | recombinant wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
0.94 | - |
(S)-malate | native wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
1.72 | - |
(S)-malate | recombinant wild type cytosolic MDH, at 25°C | Phytophthora infestans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Phytophthora infestans | 5829 | - |
mitochondrion | - |
Phytophthora infestans | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35400 | - |
subunit of mitochondrial MDH, calculated from amino acid sequence | Phytophthora infestans |
36400 | - |
subunit of cytosolic MDH, calculated from amino acid sequence | Phytophthora infestans |
41000 | - |
subunit of mitochondrial MDH, SDS-PAGE | Phytophthora infestans |
42000 | - |
subunit of cytosolic MDH, SDS-PAGE | Phytophthora infestans |
45000 | - |
2 * 45000, gel filtration | Phytophthora infestans |
90000 | - |
cytosolic MDH and mitochondrial MDH, gel filtration | Phytophthora infestans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phytophthora infestans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation and Cibacron Blue column chromatography | Phytophthora infestans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Phytophthora infestans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Phytophthora infestans | oxaloacetate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, gel filtration | Phytophthora infestans |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Phytophthora infestans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Phytophthora infestans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.25 | - |
oxaloacetate | recombinant wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
2.8 | - |
oxaloacetate | native wild type mitochondrial MDH, at 25°C | Phytophthora infestans | |
12.3 | - |
oxaloacetate | recombinant wild type cytosolic MDH, at 25°C | Phytophthora infestans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phytophthora infestans | mitochondrial MDH, calculated from amino acid sequence | - |
5.9 |
Phytophthora infestans | cytosolic MDH, calculated from amino acid sequence | - |
6.2 |