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Literature summary for 1.1.1.37 extracted from

  • Yin, Y.; Kirsch, J.F.
    Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase (2007), Proc. Natl. Acad. Sci. USA, 104, 17353-17357.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
gene mdh, expression of wild-type and mutant enzymes in strains DH10B and W945T1-2 Escherichia coli

Protein Variants

Protein Variants Comment Organism
F144I site-directed mutagenesis, inactive mutant Escherichia coli
G210A site-directed mutagenesis, the mutant shows 30% reduced activity compared to the wild-type enzyme Escherichia coli
G210A/V214I site-directed mutagenesis, the double mutant shows a 2.2fold increase in lacatate dehydrogenase activity compared to the wild-type enzyme Escherichia coli
I12V/R81Q/M85E/G210A/V214I construction of a pentamutant by site-directed mutagenesis, whose substrate specificity is switched from malate dehydrogenase to that of lactate dehydrogenase, EC 1.1.1.27, the mutant shows highly reduced activity compared to the wild-type enzyme, overview Escherichia coli
N122D site-directed mutagenesis, inactive mutant Escherichia coli
R81Q site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Escherichia coli
V214I site-directed mutagenesis, the mutant's activity is not affected Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 oxaloacetate pH 7.5, 30°C,recombinant wild-type enzyme Escherichia coli
3
-
 oxaloacetate pH 7.5, 30°C, recombinant mutant R81Q Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Escherichia coli
-
 oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+ Escherichia coli MG1655
-
 oxaloacetate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli P61889 gene mdh
-
Escherichia coli MG1655 P61889 gene mdh
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Escherichia coli oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+ residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity Escherichia coli oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+
-
 Escherichia coli MG1655 oxaloacetate + NADH + H+
-
 r
(S)-malate + NAD+ residues I12, R81, M85, G210, and V214 determine the enzyme's substrate specificity Escherichia coli MG1655 oxaloacetate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
malate dehydrogenase
-
 Escherichia coli
MDH
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.77
-
 oxaloacetate pH 7.5, 30°C, recombinant mutant R81Q Escherichia coli
931
-
 oxaloacetate pH 7.5, 30°C, recombinant wild-type enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli