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Literature summary for 1.1.1.37 extracted from
- Cloning and expression of mitochondrial malate dehydrogenase of Clonorchis sinensis (2008), Parasitol. Res., 102, 989-995.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| high throughput adult worm cDNA library screening, DNA and mino acid sequence determination and analysis, expression of the GST-tagged enzyme in Escherichia coli | Clonorchis sinensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no substrate inhibition by L-malate up to 20 mM | Clonorchis sinensis | |
| oxaloacetate | substrate inhibition at above 0.3 mM, 37% inhibition at 1 mM | Clonorchis sinensis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Clonorchis sinensis | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, recombinant GST-tagged enzyme, SDS-PAGE | Clonorchis sinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Clonorchis sinensis | the NAD-dependent mitochondrial malate dehydrogenase plays pivotal roles in tricarboxylic acid and is crucial for the survival and pathogenecity of parasites | oxaloacetate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clonorchis sinensis | Q4VVC2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography | Clonorchis sinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Clonorchis sinensis | oxaloacetate + NADH + H+ | - |
r | |
| (S)-malate + NAD+ | the NAD-dependent mitochondrial malate dehydrogenase plays pivotal roles in tricarboxylic acid and is crucial for the survival and pathogenecity of parasites | Clonorchis sinensis | oxaloacetate + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, recombinant GST-tagged enzyme, SDS-PAGE | Clonorchis sinensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| mitochondrial malate dehydrogenase | - |
Clonorchis sinensis |
| mMDH | - |
Clonorchis sinensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Clonorchis sinensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADP(H) | Clonorchis sinensis | |
| NAD+ | dependent on | Clonorchis sinensis | |
| NADH | dependent on | Clonorchis sinensis | |
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