Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | competitive with respect to oxaloacetate | Vulcanithermus mediatlanticus | |
Mn2+ | 2-40 mM | Vulcanithermus mediatlanticus | |
oxaloacetate | - |
Vulcanithermus mediatlanticus | |
Zn2+ | 0.05-0.5 mM | Vulcanithermus mediatlanticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
NADH | - |
Vulcanithermus mediatlanticus | |
0.048 | - |
oxaloacetate | - |
Vulcanithermus mediatlanticus | |
0.4 | - |
NAD+ | - |
Vulcanithermus mediatlanticus | |
1.7 | - |
malate | - |
Vulcanithermus mediatlanticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 4-40 mM, activates | Vulcanithermus mediatlanticus | |
Ca2+ | 4-40 mM, activates | Vulcanithermus mediatlanticus | |
Mg2+ | 4-40 mM, activates | Vulcanithermus mediatlanticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Vulcanithermus mediatlanticus |
128000 | - |
gel filtration | Vulcanithermus mediatlanticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vulcanithermus mediatlanticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vulcanithermus mediatlanticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.94 | - |
- |
Vulcanithermus mediatlanticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Vulcanithermus mediatlanticus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Vulcanithermus mediatlanticus | oxaloacetate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Vulcanithermus mediatlanticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
82 | - |
- |
Vulcanithermus mediatlanticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: about 45% of maximal activity, 90°C: about 60% of maximal activity | Vulcanithermus mediatlanticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, stable | Vulcanithermus mediatlanticus |
60 | - |
15 min, about 25% loss of activity | Vulcanithermus mediatlanticus |
70 | - |
15 min, about 50% loss of activity | Vulcanithermus mediatlanticus |
80 | - |
15 min, about 70% loss of activity | Vulcanithermus mediatlanticus |
85 | - |
1 min, complete loss of activity | Vulcanithermus mediatlanticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
reduction of oxaloacetate | Vulcanithermus mediatlanticus |
8.5 | - |
oxidation of malate | Vulcanithermus mediatlanticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0205 | - |
citrate | - |
Vulcanithermus mediatlanticus | |
0.37 | - |
Zn2+ | - |
Vulcanithermus mediatlanticus | |
2.1 | - |
oxaloacetate | - |
Vulcanithermus mediatlanticus | |
2.5 | - |
Mn2+ | - |
Vulcanithermus mediatlanticus |