Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-malate | - |
Sus scrofa | |
L-malate | - |
Comamonas testosteroni |
Crystallization (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
- |
Delftia acidovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxaloacetate | - |
Comamonas testosteroni | |
oxaloacetate | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
NADH | - |
Comamonas testosteroni | |
0.057 | - |
oxaloacetate | - |
Comamonas testosteroni | |
0.069 | - |
L-malate | 0.03-0.1 mM substrate | Comamonas testosteroni | |
0.089 | - |
3-acetylnicotinamide-NAD+ | - |
Comamonas testosteroni | |
0.109 | - |
NAD+ | - |
Comamonas testosteroni | |
0.23 | - |
thionicotinamide-NAD+ | - |
Comamonas testosteroni | |
0.263 | - |
L-malate | 0.2-10 mM substrate | Comamonas testosteroni | |
0.264 | - |
deamino-NAD+ | - |
Comamonas testosteroni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
mitochondrion | - |
Bos taurus | 5739 | - |
mitochondrion | - |
Equus sp. | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33500 | - |
4 * 33500, SDS-PAGE | Bacillus subtilis |
36000 | - |
2 * 36000, SDS-PAGE | Comamonas testosteroni |
43000 | - |
sedimentation equilibrium centrifugation | Delftia acidovorans |
60000 | 67000 | sedimentation equilibrium centrifugation | Escherichia coli |
70000 | - |
gel filtration, analytical Sephadex | Comamonas testosteroni |
74200 | - |
sedimentation equilibrium centrifugation | Comamonas testosteroni |
75000 | - |
Svedberg equation | Comamonas testosteroni |
117000 | 148000 | sedimentation equilibrium centrifugation | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | Bacillus subtilis | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Escherichia coli | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Bos taurus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Triticum aestivum | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Geobacillus stearothermophilus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Solanum tuberosum | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Comamonas testosteroni | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Delftia acidovorans | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Equus sp. | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bos taurus | - |
ox | - |
Comamonas testosteroni | - |
Stanier strain 78, ATCC 11996 | - |
Delftia acidovorans | - |
- |
- |
Equus sp. | - |
- |
- |
Escherichia coli | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Solanum tuberosum | - |
potato | - |
Sus scrofa | - |
pig | - |
Triticum aestivum | - |
wheat | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
- |
Escherichia coli |
- |
Geobacillus stearothermophilus |
- |
Comamonas testosteroni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
germ | - |
Triticum aestivum | - |
heart | - |
Sus scrofa | - |
heart | - |
Bos taurus | - |
heart | - |
Equus sp. | - |
tuber | - |
Solanum tuberosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.762 | - |
- |
Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-tartrate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
r | |
(S)-2-hydroxyglutarate + NAD+ | oxidized at an extremely slow rate, less than 0.001% of the rate of L-malate oxidation | Comamonas testosteroni | 2-oxoglutarate + NADH + H+ | - |
r | |
(S)-malate + 3-acetylnicotinamide-NAD+ | - |
Comamonas testosteroni | oxaloacetate + 3-acetylnicotinamide-NADH + H+ | - |
r | |
(S)-malate + deamino-NAD+ | - |
Comamonas testosteroni | oxaloacetate + deamino-NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Triticum aestivum | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Geobacillus stearothermophilus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Solanum tuberosum | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Comamonas testosteroni | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Delftia acidovorans | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Equus sp. | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + thionicotinamide-NAD+ | - |
Comamonas testosteroni | oxaloacetate + thionicotinamide-NADH + H+ | - |
r | |
alpha-keto-malonate + NADH | - |
Sus scrofa | ? + NAD+ | - |
? | |
alpha-keto-malonate + NADH | - |
Comamonas testosteroni | ? + NAD+ | - |
ir | |
hydroxymalonate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
? | |
malate + NAD+ | - |
Bacillus subtilis | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Bos taurus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Triticum aestivum | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Geobacillus stearothermophilus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Solanum tuberosum | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Comamonas testosteroni | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Delftia acidovorans | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Equus sp. | oxaloacetate + NADH + H+ | - |
r | |
meso-tartrate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
r | |
additional information | D-malate, (+)-tartrate, succinate and L-aspartate are not oxidized | Comamonas testosteroni | ? | - |
? | |
oxaloacetate + NADH | - |
Bacillus subtilis | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Escherichia coli | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Sus scrofa | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Bos taurus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Triticum aestivum | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Geobacillus stearothermophilus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Solanum tuberosum | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Comamonas testosteroni | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Delftia acidovorans | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Equus sp. | L-malate + NAD+ | - |
r | |
oxaloglycolate + NAD+ | - |
Comamonas testosteroni | ? + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Sus scrofa |
dimer | - |
Bos taurus |
dimer | 2 * 36000, SDS-PAGE | Comamonas testosteroni |
tetramer | - |
Escherichia coli |
tetramer | - |
Geobacillus stearothermophilus |
tetramer | 4 * 33500, SDS-PAGE | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
oxaloacetate reduction | Comamonas testosteroni |
10.4 | - |
malate oxidation | Comamonas testosteroni |