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Literature summary for 1.1.1.368 extracted from

  • Laempe, D.; Jahn, M.; Fuchs, G.
    6-Hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, enzymes of the benzoyl-CoA pathway of anaerobic aromatic metabolism in the denitrifying bacterium Thauera aromatica (1999), Eur. J. Biochem., 263, 420-429.
    View publication on PubMed
Search for more literature for 1.1.1.368

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Thauera aromatica

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzymew is insensitive to 10 mM EDTA, 1 mM 2,2'-bipyridyl, or 1 mM pyrazole Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 6-hydroxycyclohex-1-ene-1-carbonyl-CoA pH 7.4, 37°C Thauera aromatica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is not increased after adding 2-20 mM of ZnCl2 Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, SDS-PAGE Thauera aromatica
78000
-
 gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ Thauera aromatica the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Thauera aromatica O87871
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thauera aromatica

Source Tissue

Source Tissue Comment Organism Textmining
culture condition when cells are grown aerobically with benzoate the activity is 20-25fold lower than in cells grown anaerobically with benzoate Thauera aromatica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.8
-
 pH 7.4, 37°C, substrate: 6-hydroxycyclohex-1-ene-1-carbonyl-CoA Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria Thauera aromatica 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+
-
 ir
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ the enzyme is specific for NAD+, no activity with NADP+. The enzyme is specific for 6-hydroxycyclohex-1-ene-1-carbonyl-CoA, no activity with 2-hydroxycyclohexane-1-carbonyl-CoA Thauera aromatica 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+
-
 ir

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Thauera aromatica

Synonyms

Synonyms Comment Organism
HAD
-
 Thauera aromatica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Thauera aromatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7
-
 6-hydroxycyclohex-1-ene-1-carbonyl-CoA pH 7.4, 37°C, calculated as kcat number per subunit Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Thauera aromatica
7.4
-
 assay at Thauera aromatica

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme is specific for NAD+, no activity with NADP+ Thauera aromatica

Expression

Organism Comment Expression
Thauera aromatica expression of the enzyme activity is under negative control by oxygen down
Thauera aromatica gene expression is positively controlled by benzoate, an intermediate of the central benzoyl-CoA pathway up