Literature summary for 1.1.1.365 extracted from

Matsubara, T.; Hamada, S.; Wakabayashi, A.; Kishida, M.
Fermentative production of L-galactonate by using recombinant Saccharomyces cerevisiae containing the endogenous galacturonate reductase gene from Cryptococcus diffluens (2016), J. Biosci. Bioeng., 122, 639-644 .

Search for more literature for 1.1.1.365

Cloned(Commentary)

Cloned (Comment)	Organism
gene GAR1, DNA and amino acid sequence determination and analysis, functional recombinant expression in Saccharomyces cerevisiae strain IFO 10455 from a genome integrated copy, integration of the pGK406GAR1 plasmid into the ura3 allele of the IFO 10455 genome, subcloning in Escherichia coli strain DH5alpha	Naganishia diffluens

Protein Variants

Protein Variants	Comment	Organism
additional information	development of an ethanol fermentation system from D-galacturonic acid (or pectic waste) in enzyme lacking Saccharomyces cerevisiae, brewing yeast. Optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview. High efficiency in the conversion of D-galacturonic acid to L-galactonic acid in large-scale cultures is achieved with 0.1% initial D-galacturonic acid concentration, pH 3.5, and glucose as additional sugar, aerobic condition is necessary. Subculture of this recombinant is not showing to decrease of the D-galacturonic acid conversion rate even though it is repeated in ten generations. Culturing in scale-up, the conversion rate of D-galacturonic acid to L-galactonic acid is increased. The recombinant strain, similar to its wild-type host strain IFO 10455, cannot grow in media containing D-galacturonic acid as the sole carbon source, but it can grow after the addition of Saccharomyces cerevisiae fermentative sugars like glucose and galactose	Naganishia diffluens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-galacturonate + NADPH + H+	Naganishia diffluens	-	L-galactonate + NADP+	-	r
D-galacturonate + NADPH + H+	Naganishia diffluens OPU-FC11	-	L-galactonate + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Naganishia diffluens	HV538330	a D-galacturonic acid-utilizing, psychrophilic yeast strain	-
Naganishia diffluens OPU-FC11	HV538330	a D-galacturonic acid-utilizing, psychrophilic yeast strain	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-galacturonate + NADPH + H+	-	Naganishia diffluens	L-galactonate + NADP+	-	r
D-galacturonate + NADPH + H+	-	Naganishia diffluens OPU-FC11	L-galactonate + NADP+	-	r
additional information	optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview	Naganishia diffluens	?	-	?
additional information	optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview	Naganishia diffluens OPU-FC11	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 46450, sequence calculation, x * 45000, recombinant enzyme, SDS-PAGE	Naganishia diffluens

Synonyms

Synonyms	Comment	Organism
Cd-GAR	-	Naganishia diffluens
galacturonate reductase	-	Naganishia diffluens
gar1	-	Naganishia diffluens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.5	-	in vivo activity	Naganishia diffluens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Naganishia diffluens
NADPH	-	Naganishia diffluens

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the NAD(P)+-binding Rossmann fold oxidoreductase family of proteins	Naganishia diffluens

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Release 2023.1 (January 2023)