Cloned (Comment) | Organism |
---|---|
gene GAR1, DNA and amino acid sequence determination and analysis, functional recombinant expression in Saccharomyces cerevisiae strain IFO 10455 from a genome integrated copy, integration of the pGK406GAR1 plasmid into the ura3 allele of the IFO 10455 genome, subcloning in Escherichia coli strain DH5alpha | Naganishia diffluens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of an ethanol fermentation system from D-galacturonic acid (or pectic waste) in enzyme lacking Saccharomyces cerevisiae, brewing yeast. Optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview. High efficiency in the conversion of D-galacturonic acid to L-galactonic acid in large-scale cultures is achieved with 0.1% initial D-galacturonic acid concentration, pH 3.5, and glucose as additional sugar, aerobic condition is necessary. Subculture of this recombinant is not showing to decrease of the D-galacturonic acid conversion rate even though it is repeated in ten generations. Culturing in scale-up, the conversion rate of D-galacturonic acid to L-galactonic acid is increased. The recombinant strain, similar to its wild-type host strain IFO 10455, cannot grow in media containing D-galacturonic acid as the sole carbon source, but it can grow after the addition of Saccharomyces cerevisiae fermentative sugars like glucose and galactose | Naganishia diffluens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | Naganishia diffluens | - |
L-galactonate + NADP+ | - |
r | |
D-galacturonate + NADPH + H+ | Naganishia diffluens OPU-FC11 | - |
L-galactonate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Naganishia diffluens | HV538330 | a D-galacturonic acid-utilizing, psychrophilic yeast strain | - |
Naganishia diffluens OPU-FC11 | HV538330 | a D-galacturonic acid-utilizing, psychrophilic yeast strain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | - |
Naganishia diffluens | L-galactonate + NADP+ | - |
r | |
D-galacturonate + NADPH + H+ | - |
Naganishia diffluens OPU-FC11 | L-galactonate + NADP+ | - |
r | |
additional information | optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview | Naganishia diffluens | ? | - |
? | |
additional information | optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview | Naganishia diffluens OPU-FC11 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46450, sequence calculation, x * 45000, recombinant enzyme, SDS-PAGE | Naganishia diffluens |
Synonyms | Comment | Organism |
---|---|---|
Cd-GAR | - |
Naganishia diffluens |
galacturonate reductase | - |
Naganishia diffluens |
gar1 | - |
Naganishia diffluens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
in vivo activity | Naganishia diffluens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Naganishia diffluens | |
NADPH | - |
Naganishia diffluens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the NAD(P)+-binding Rossmann fold oxidoreductase family of proteins | Naganishia diffluens |