Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain CEN.PK2-1B | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NADP+ | in 100 mM sodium phosphate (pH 7.0), at 22°C | Trichoderma reesei | |
0.03 | - |
NADPH | in 100 mM sodium phosphate (pH 7.0), at 22°C | Trichoderma reesei | |
4 | - |
L-galactonate | in 100 mM sodium phosphate (pH 7.0), at 22°C | Trichoderma reesei | |
6 | - |
DL-glyceraldehyde | in 100 mM sodium phosphate (pH 7.0), at 22°C | Trichoderma reesei | |
6 | - |
D-galacturonate | in 100 mM sodium phosphate (pH 7.0), at 22°C | Trichoderma reesei | |
11 | - |
D-glucuronic acid | in 100 mM sodium phosphate (pH 7.0), at 22°C | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | - |
- |
- |
Trichoderma reesei RUT C-30 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Fractogel TSK DEAE-650 column chromatography | Trichoderma reesei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:D-galacturonate-grown cell | - |
Trichoderma reesei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | - |
Trichoderma reesei | L-galactonate + NADP+ | - |
r | |
D-galacturonate + NADPH + H+ | - |
Trichoderma reesei RUT C-30 | L-galactonate + NADP+ | - |
r | |
D-glucuronic acid + NADPH + H+ | - |
Trichoderma reesei | ? | - |
? | |
D-glucuronic acid + NADPH + H+ | - |
Trichoderma reesei RUT C-30 | ? | - |
? | |
DL-glyceraldehyde + NADPH + H+ | - |
Trichoderma reesei | ? | - |
? | |
DL-glyceraldehyde + NADPH + H+ | - |
Trichoderma reesei RUT C-30 | ? | - |
? | |
L-galactonate + NADP+ | - |
Trichoderma reesei | D-galacturonate + NADPH + H+ | - |
r | |
L-galactonate + NADP+ | - |
Trichoderma reesei RUT C-30 | D-galacturonate + NADPH + H+ | - |
r | |
additional information | no activity with NADH and D-glucose, D-fructose, D-xylose, D-galactose, L-arabinose, or D-mannose. No backward reaction with L-gulonic acid, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol | Trichoderma reesei | ? | - |
? | |
additional information | no activity with NADH and D-glucose, D-fructose, D-xylose, D-galactose, L-arabinose, or D-mannose. No backward reaction with L-gulonic acid, glycerol, D-arabitol, L-arabitol, xylitol, galactitol, or ribitol | Trichoderma reesei RUT C-30 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
D-galacturonic acid reductase | - |
Trichoderma reesei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Trichoderma reesei |