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Literature summary for 1.1.1.364 extracted from

  • Thuy, T.T.; Liou, K.; Oh, T.J.; Kim, D.H.; Nam, D.H.; Yoo, J.C.; Sohng, J.K.
    Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP (2007), Glycobiology, 17, 119-126.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, including the 6His-tag and thioredoxin-tagged fusion protein, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-4-dehydro-6-deoxy-alpha-D-gulopyranose + NADH + H+ Streptomyces sp. the enzyme forms an activated deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II dTDP-6-deoxy-alpha-D-allopyranose + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. Q331Q7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-6-deoxy-alpha-D-gulopyranose + NADH + H+ the enzyme forms an activated deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II Streptomyces sp. dTDP-6-deoxy-alpha-D-allopyranose + NAD+
-
 ?
dTDP-4-dehydro-6-deoxy-alpha-D-gulopyranose + NADH + H+ dTDP-4-dehydro-6-deoxy-alpha-D-gulose i.e. dTDP-4-dehydro-6-deoxy-alpha-D-allose Streptomyces sp. dTDP-6-deoxy-alpha-D-allopyranose + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, including the 6His-tag and thioredoxin-tagged fusion protein, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
GerKI
-
 Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Streptomyces sp.

General Information

General Information Comment Organism
physiological function the enzyme forms an activated deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II Streptomyces sp.