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Literature summary for 1.1.1.363 extracted from

  • Cosgrove, M.S.; Naylor, C.; Paludan, S.; Adams, M.J.; Levy, H.R.
    On the mechanism of the reaction catalyzed by glucose 6-phosphate dehydrogenase (1998), Biochemistry, 37, 2759-2767.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
the three-dimensional structure of the H240N glucose 6-phosphate dehydrogenase is determined at 2.5 A resolution. Crystals are grown in the presence of either glucose 6-phosphate and NAD+ or glucose 6-phosphate and NADP+, hanging drop vapor diffusion method with 2.27 M ammonium sulfate Leuconostoc mesenteroides

Protein Variants

Protein Variants Comment Organism
D177N kcat/KM for D-glucose 6-phosphate (with cosubstrate NADP+) is fold lower than the value for the wild-type enzyme, kcat/KM for NADP+ is fold lower than the value for the wild-type enzyme, kcat/KM for D-glucose 6-phosphate (with cosubstrate NAD) is fold lower than the value for the wild-type enzyme, kcat/KM for D-glucose 6-phosphate (with cosubstrate NAD+) is fold lower than the value for the wild-type enzyme Leuconostoc mesenteroides
H178N kcat/KM for D-glucose 6-phosphate (with cosubstrate NADP+) is fold lower than the value for the wild-type enzyme, kcat/KM for NADP+ is fold lower than the value for the wild-type enzyme, kcat/KM for D-glucose 6-phosphate (with cosubstrate NAD) is fold lower than the value for the wild-type enzyme, kcat/KM for D-glucose 6-phosphate (with cosubstrate NAD+) is fold lower than the value for the wild-type enzyme Leuconostoc mesenteroides
H250N kcat/KM for D-glucose 6-phosphate (with cosubstrate NADP+) is fold lower than the value for the wild-type enzyme, kcat/KM for NADP+ is fold lower than the value for the wild-type enzyme, kcat/KM for D-glucose 6-phosphate (with cosubstrate NAD) is fold lower than the value for the wild-type enzyme, kcat/KM for D-glucose 6-phosphate (with cosubstrate NAD+) is fold lower than the value for the wild-type enzyme Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
NADP+
-
Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
NADP+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides
0.0088
-
NADP+ 25°C, pH 7.6, mutant enzyme H240N Leuconostoc mesenteroides
0.0152
-
NADP+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides
0.117
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme D177N Leuconostoc mesenteroides
0.29
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme D177N Leuconostoc mesenteroides
0.8
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme H240N Leuconostoc mesenteroides
0.81
-
NAD+ 25°C, pH 7.6, mutant enzyme H240N Leuconostoc mesenteroides
0.95
-
NAD+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides
1.14
-
NAD+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides
1.28
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme H240N Leuconostoc mesenteroides
13.6
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme H1787N Leuconostoc mesenteroides
42.9
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme H1787N Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + NAD+ Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose 6-phosphate + NADP+ Leuconostoc mesenteroides
-
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes H250N, D177N and H178N Leuconostoc mesenteroides

Reaction

Reaction Comment Organism Reaction ID
D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ mechanism in which His240 acts as the general base that abstracts the proton from the C1-hydroxyl group of glucose 6-phosphate, and the carboxylate group of D177 stabilizes the positive charge that forms on H240 in the transition state. The results also confirm the postulated role of His178 in binding the phosphate moiety of glucose 6-phosphate Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NAD+
-
Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADH + H+
-
?
D-glucose 6-phosphate + NADP+
-
Leuconostoc mesenteroides 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Leuconostoc mesenteroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme H240N Leuconostoc mesenteroides
0.007
-
NADP+ 25°C, pH 7.6, mutant enzyme H240N Leuconostoc mesenteroides
0.02
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme H240N Leuconostoc mesenteroides
0.02
-
NAD+ 25°C, pH 7.6, mutant enzyme H240N Leuconostoc mesenteroides
1.1
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme D177N Leuconostoc mesenteroides
1.1
-
NADP+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides
4.85
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme D177N Leuconostoc mesenteroides
4.85
-
NAD+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides
13.8
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme H1787N Leuconostoc mesenteroides
13.8
-
NADP+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides
14.2
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme H1787N Leuconostoc mesenteroides
14.2
-
NAD+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Leuconostoc mesenteroides

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0037
-
NADP+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides
0.015
-
NADP+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme H240N Leuconostoc mesenteroides
0.025
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme H240N Leuconostoc mesenteroides
0.025
-
NAD+ 25°C, pH 7.6, mutant enzyme H240N Leuconostoc mesenteroides
0.32
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme H1787N Leuconostoc mesenteroides
0.8
-
NADP+ 25°C, pH 7.6, mutant enzyme H240N Leuconostoc mesenteroides
1.04
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme H1787N Leuconostoc mesenteroides
4.25
-
NAD+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides
9.4
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NADP+, mutant enzyme D177N Leuconostoc mesenteroides
14.95
-
NAD+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides
16.7
-
D-glucose 6-phosphate 25°C, pH 7.6, cosubstrate: NAD+, mutant enzyme D177N Leuconostoc mesenteroides
134.1
-
NADP+ 25°C, pH 7.6, mutant enzyme D177N Leuconostoc mesenteroides
904
-
NADP+ 25°C, pH 7.6, mutant enzyme H1787N Leuconostoc mesenteroides