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Literature summary for 1.1.1.36 extracted from

  • Rodarte, J.V.; Abendroth, J.; Edwards, T.E.; Lorimer, D.D.; Staker, B.L.; Zhang, S.; Myler, P.J.; McLaughlin, K.J.
    Crystal structure of acetoacetyl-CoA reductase from Rickettsia felis (2021), Acta Crystallogr. Sect. F, 77, 54-60 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rickettsia felis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 10% (w/v) PEG 8000, 8% (w/v) ethylene glycol, 100 mM HEPES-NaOH pH 7.5 Rickettsia felis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101000
-
gel filtration Rickettsia felis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-oxoacyl-CoA + NADPH + H+ Rickettsia felis
-
(R)-3-hydroxyacyl-CoA + NADP+
-
r
3-oxoacyl-CoA + NADPH + H+ Rickettsia felis ATCC VR-1525
-
(R)-3-hydroxyacyl-CoA + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Rickettsia felis Q4UN54
-
-
Rickettsia felis ATCC VR-1525 Q4UN54
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Rickettsia felis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-oxoacyl-CoA + NADPH + H+
-
Rickettsia felis (R)-3-hydroxyacyl-CoA + NADP+
-
r
3-oxoacyl-CoA + NADPH + H+
-
Rickettsia felis ATCC VR-1525 (R)-3-hydroxyacyl-CoA + NADP+
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 26000, SDS-PAGE Rickettsia felis
homotetramer 4 * 25700, calculated from amino acid sequence Rickettsia felis

Synonyms

Synonyms Comment Organism
AcAc-CoA reductase
-
Rickettsia felis
PhbB
-
Rickettsia felis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Rickettsia felis