Literature summary for 1.1.1.36 extracted from

Ylianttila, M.S.; Qin, Y.M.; Hiltunen, J.K.; Glumoff, T.
Site-directed mutagenesis to enable and improve crystallizability of Candida tropicalis (3R)-hydroxyacyl-CoA dehydrogenase (2004), Biochem. Biophys. Res. Commun., 324, 25-30.

Cloned(Commentary)

Cloned (Comment)	Organism
N-terminal part of amino acids 1-604 is expressed as a C-terminally His-tagged, stable protein in Escherichia coli strain BL21(DE3)	Candida tropicalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
5 mg/ml purified recombinant, detagged truncated enzyme, amino acid residues 1-604, by vapor diffusion technique, 20°C, in 30% PEG 4000, 0.1 M sodium acetate, pH 4.2, 0.2 M ammonium acetate, equal volume of 0.002 ml of protein and precipitant solution, 11% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.22-2.36 A resolution, the wild-type enzyme, comprising amino acid residues 1-906, is unstable and not crystallizable	Candida tropicalis

Crystallization (Comment)

Organism

5 mg/ml purified recombinant, detagged truncated enzyme, amino acid residues 1-604, by vapor diffusion technique, 20°C, in 30% PEG 4000, 0.1 M sodium acetate, pH 4.2, 0.2 M ammonium acetate, equal volume of 0.002 ml of protein and precipitant solution, 11% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 2.22-2.36 A resolution, the wild-type enzyme, comprising amino acid residues 1-906, is unstable and not crystallizable

Candida tropicalis

Protein Variants

Protein Variants	Comment	Organism
additional information	site-directed mutagenesis, while the wild-type enzyme, comprising amino acid residues 1-906, is unstable and not crystallizable, as is the recombinant truncated version comprising amino acid residues 1-591, the mutants 1-604 and 1-612, with or without further modifications, are stable with different crystallizability, overview	Candida tropicalis

Organism

Organism	UniProt	Comment	Textmining
Candida tropicalis	-	multifunctional enzyme type 2, i.e. MFE-2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged, truncated enzyme from Escherichia coli, the His-tag is cleaved off by factor Xa	Candida tropicalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the N-terminal enzyme part harbors 2 different (3R)-hydroxyacyl-CoA dehydrogenase activities with different substrate specificities, while the 2-enoyl-CoA hydratase 2 activity is located in the C-terminus	Candida tropicalis	?	-	?

Synonyms

Synonyms	Comment	Organism
(3R)-hydroxyacyl-CoA dehydrogenase	-	Candida tropicalis
MFE-2	-	Candida tropicalis