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Literature summary for 1.1.1.359 extracted from

  • Milburn, C.C.; Lamble, H.J.; Theodossis, A.; Bull, S.D.; Hough, D.W.; Danson, M.J.; Taylor, G.L.
    The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus (2006), J. Biol. Chem., 281, 14796-14804.
    View publication on PubMed
Search for more literature for 1.1.1.359

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop, vapor-diffusion method, crystal structure of the apo form of glucose dehydrogenase to a resolution of 1.8 A and a complex with its required cofactor, NADP+, to a resolution of 2.3 A. Complexes of the enzyme with D-glucose and D-xylose are presented to resolutions of 1.6 and 1.5 A. A T41A mutation is engineered to enable the trapping of substrate in the crystal Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
T41A kcat/Km value of the mutant enzyme for beta-D-glucose (with NAD+) is about 30fold lower compared to wild-type enzyme, kcat/Km value of the mutant enzyme for beta-D-flucose (with NADP+) is about 90fold lower compared to wild-type enzyme Saccharolobus solfataricus
T41V kcat/Km value of the mutant enzyme for beta-D-glucose (with NAD+) is about 555fold lower compared to wild-type enzyme, kcat/Km value of the mutant enzyme for beta-D-flucose (with NADP+) is about 530fold lower compared to wild-type enzyme Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
0.25
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
0.44
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
0.57
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
1.3
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
1.5
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
20.4
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
24.8
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
29.2
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
33.3
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
59
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
65.8
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
72.5
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
76.3
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
109
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
118
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
175
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
204
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus O93715
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD+ + H2O
-
 Saccharolobus solfataricus D-gluconate + NADH + 2 H+
-
 ir
beta-D-glucose + NADP+ + H2O
-
 Saccharolobus solfataricus D-gluconate + NADPH + 2 H+
-
 ir
D-galactose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
D-galactose + NADP+ + H2O
-
 Saccharolobus solfataricus D-galactonate + NADPH + 2 H+
-
 ?
D-xylose + NAD+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
D-xylose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
6
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
7
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
7
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
8
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
12
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
14
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
22
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
22
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
37
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
39
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
44
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
48
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
56
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
61
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
61
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
75
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
81
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.04
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
0.04
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
0.07
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
0.09
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
0.11
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41V Saccharolobus solfataricus
0.15
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41V Saccharolobus solfataricus
0.2
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
0.4
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
0.5
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
1.1
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, mutant enzyme T41A Saccharolobus solfataricus
1.6
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
2.8
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, mutant enzyme T41A Saccharolobus solfataricus
37
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
50
-
 beta-D-glucose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
85
-
 D-galactose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus
108
-
 D-galactose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
245
-
 D-xylose pH 7.5, 70°C, cosubstrate: NAD+, wild-type enzyme Saccharolobus solfataricus
246
-
 D-xylose pH 7.5, 70°C, cosubstrate: NADP+, wild-type enzyme Saccharolobus solfataricus