Crystallization (Comment) | Organism |
---|---|
- |
Candida parapsilosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-dehydropantolactone | competitive versus isatin | Candida parapsilosis | |
3,4-Dihydroxy-3-cyclobutene-1,2-dione | uncompetitive | Candida parapsilosis | |
Al3+ | 83%% inhibition, reduction of 2-dehydropantolactone | Candida parapsilosis | |
Cd2+ | 97% inhibition, reduction of 2-dehydropantolactone | Candida parapsilosis | |
Cu2+ | completely inhibits reduction of 2-dehydropantolactone | Candida parapsilosis | |
cyclohexenediol-1,2,3,4-tetraone | uncompetitive | Candida parapsilosis | |
Hg2+ | completely inhibits reduction of 2-dehydropantolactone | Candida parapsilosis | |
iodoacetate | 1 mM, 49% inhibition | Candida parapsilosis | |
p-chloromercuribenzoate | 0.1 mM, 72% inhibition | Candida parapsilosis | |
parabanic acid | uncompetitive | Candida parapsilosis | |
quercetin | 0.1 mM, 94% inhibition | Candida parapsilosis | |
Zn2+ | completely inhibits reduction of 2-dehydropantolactone | Candida parapsilosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
isatin | pH 7.0, 30°C | Candida parapsilosis | |
0.016 | - |
5-methylisatin | pH 7.0, 30°C | Candida parapsilosis | |
0.085 | - |
1-methylisatin | pH 7.0, 30°C | Candida parapsilosis | |
0.333 | - |
2-dehydropantolactone | pH 7.0, 30°C | Candida parapsilosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Candida parapsilosis |
40000 | - |
polyacrylamide gel electrophoresis | Candida parapsilosis |
41600 | - |
1 * 41600, SDS-PAGE | Candida parapsilosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydropantolactone + NADPH + H+ | Candida parapsilosis | - |
(R)-pantolactone + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | - |
- |
- |
Candida parapsilosis IFO 0708 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida parapsilosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
173 | - |
pH 7.0, 30°C, substrate: 2-dehydropantolactone | Candida parapsilosis |
251 | - |
pH 7.0, 30°C, substrate: isatin | Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methylisatin + NADPH + H+ | 86% of the activity compared to isatin | Candida parapsilosis | ? | - |
? | |
2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis | (R)-pantolactone + NADP+ | - |
ir | |
2-dehydropantolactone + NADPH + H+ | 69% of the activity compared to isatin. The stereospecificity for NADPH is not known. Activity with NADH is 2% compared to the activity with NADPH | Candida parapsilosis | (R)-pantolactone + NADP+ | - |
ir | |
5-methylisatin + NADPH + H+ | 108% of the activity compared to isatin | Candida parapsilosis | ? | - |
? | |
camphorquinone + NADPH + H+ | 97% of the activity compared to isatin | Candida parapsilosis | ? | - |
? | |
isatin + NADPH + H+ | - |
Candida parapsilosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41600, SDS-PAGE | Candida parapsilosis |
Synonyms | Comment | Organism |
---|---|---|
ketopantoyl-lactone reductase | - |
Candida parapsilosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.0, 10 min, 58% loss of activity | Candida parapsilosis |
60 | - |
pH 7.0, 10 min, 77% loss of activity | Candida parapsilosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
30°C, 30 min, 33% loss of activity | Candida parapsilosis |
6 | 10 | enzyme is stable | Candida parapsilosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | activity with NADH is 2% compared to the activity with NADPH | Candida parapsilosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
cyclohexenediol-1,2,3,4-tetraone | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
0.0014 | - |
3,4-Dihydroxy-3-cyclobutene-1,2-dione | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
0.19 | - |
2-dehydropantolactone | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis | |
3.14 | - |
parabanic acid | pH 7.0, 30°C, substrate: isatin | Candida parapsilosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candida parapsilosis | electrofocussing | - |
6.3 |