BRENDA - Enzyme Database
show all sequences of 1.1.1.355

The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK

Sucipto, H.; Kudo, F.; Eguchi, T.; Angew. Chem. Int. Ed. Engl. 51, 3428-3431 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces kanamyceticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2'-dehydrokanamycin A + NADPH + H+
Streptomyces kanamyceticus
the enzyme is involved in the conversion of kanamycin B to kanamycin A. Kanamycin is a clinically important aminoglycoside antibiotic
kanamycin A + NADP+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces kanamyceticus
Q2MFU6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces kanamyceticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2'-dehydrokanamycin A + NADPH + H+
the enzyme is involved in the conversion of kanamycin B to kanamycin A. Kanamycin is a clinically important aminoglycoside antibiotic
721287
Streptomyces kanamyceticus
kanamycin A + NADP+
-
-
-
?
2'-dehydrokanamycin A + NADPH + H+
i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-a-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside
721287
Streptomyces kanamyceticus
kanamycin A + NADP+
i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-[(6-amino-6-deoxy-a-D-arabino-hexopyranosyl-2-ulose)oxy]-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside
-
-
?
Synonyms
Synonyms
Commentary
Organism
kanK
-
Streptomyces kanamyceticus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Streptomyces kanamyceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces kanamyceticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Streptomyces kanamyceticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2'-dehydrokanamycin A + NADPH + H+
Streptomyces kanamyceticus
the enzyme is involved in the conversion of kanamycin B to kanamycin A. Kanamycin is a clinically important aminoglycoside antibiotic
kanamycin A + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces kanamyceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2'-dehydrokanamycin A + NADPH + H+
the enzyme is involved in the conversion of kanamycin B to kanamycin A. Kanamycin is a clinically important aminoglycoside antibiotic
721287
Streptomyces kanamyceticus
kanamycin A + NADP+
-
-
-
?
2'-dehydrokanamycin A + NADPH + H+
i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-a-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside
721287
Streptomyces kanamyceticus
kanamycin A + NADP+
i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-[(6-amino-6-deoxy-a-D-arabino-hexopyranosyl-2-ulose)oxy]-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside
-
-
?
Other publictions for EC 1.1.1.355
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721287
Sucipto
The last step of kanamycin bio ...
Streptomyces kanamyceticus
Angew. Chem. Int. Ed. Engl.
51
3428-3431
2012
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