Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.353 extracted from

  • Matsushima, K.; Ando, Y.; Hamasaki, T.; Yabe, K.
    Purification and characterization of two versiconal hemiacetal acetate reductases involved in aflatoxin biosynthesis (1994), Appl. Environ. Microbiol., 60, 2561-2567.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.353

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0254
-
 versiconal hemiacetal acetate isoform VHA reductase II, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C Aspergillus parasiticus
0.0354
-
 versiconal hemiacetal acetate isoform VHA reductase I, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C Aspergillus parasiticus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Aspergillus parasiticus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 10 * 39000, isoform VHA reductase I, SDS-PAGE Aspergillus parasiticus
40000
-
 10 * 40000, isoform VHA reductase II, SDS-PAGE Aspergillus parasiticus
390000
-
 gel filtration Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
versiconal hemiacetal acetate + NADPH + H+ Aspergillus parasiticus both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced versiconol acetate + NADP+
-
 r
versiconal hemiacetal acetate + NADPH + H+ Aspergillus parasiticus NUIH-26 both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced versiconol acetate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Aspergillus parasiticus
-
-
-
Aspergillus parasiticus NUIH-26
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, Sephacryl S-300 gel filtration, hydroxylapatite column chromatography, and Matrex gel Green A chromatography Aspergillus parasiticus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus parasiticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00362
-
 crude extract, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C Aspergillus parasiticus
0.027
-
 isoform VHA reductase II, after 7.5fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C Aspergillus parasiticus
0.052
-
 isoform VHA reductase I, after 14.4fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 37°C Aspergillus parasiticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
versiconal hemiacetal acetate + NADPH + H+ both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced Aspergillus parasiticus versiconol acetate + NADP+
-
 r
versiconal hemiacetal acetate + NADPH + H+ both (2'S)- and (2'R)-versiconol acetate enantiomers are formed from racemic versiconal hemiacetal acetate and more of the (2'R)- isomer (72-88%) than the (2'S)-isomer (12-28%) is produced Aspergillus parasiticus NUIH-26 versiconol acetate + NADP+
-
 r

Subunits

Subunits Comment Organism
homodecamer 10 * 39000, isoform VHA reductase I, SDS-PAGE Aspergillus parasiticus
homodecamer 10 * 40000, isoform VHA reductase II, SDS-PAGE Aspergillus parasiticus

Synonyms

Synonyms Comment Organism
VHA reductase I
-
 Aspergillus parasiticus
VHA reductase II
-
 Aspergillus parasiticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 isoform VHA reductase I Aspergillus parasiticus
9
-
 isoform VHA reductase II Aspergillus parasiticus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 no activity is detected below pH 4.0 Aspergillus parasiticus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Aspergillus parasiticus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus parasiticus isoform VHA reductase II, isoelectric focusing
-
 6
Aspergillus parasiticus isoform VHA reductase I, isoelectric focusing
-
 6.6