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Literature summary for 1.1.1.352 extracted from
- Involvement of two cytosolic enzymes and a novel intermediate, 5-oxoaverantin, in the pathway from 5-hydroxyaverantin to averufin in aflatoxin biosynthesis (2003), Appl. Environ. Microbiol., 69, 6418-6426.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
5'-hydroxyaverantin | in 60 mM potassium phosphate buffer (pH 7.5), at 30°C | Aspergillus parasiticus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Aspergillus parasiticus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
2 * 28000, SDS-PAGE | Aspergillus parasiticus |
| 29600 | - |
2 * 29600, calculated from amino acid sequence | Aspergillus parasiticus |
| 60000 | - |
gel filtration | Aspergillus parasiticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-hydroxyaverantin + NAD+ | Aspergillus parasiticus | - |
5'-oxoaverantin + NADH + H+ | - |
? | |
| 5'-hydroxyaverantin + NAD+ | Aspergillus parasiticus NIAH-26 | - |
5'-oxoaverantin + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus parasiticus | - |
- |
- |
| Aspergillus parasiticus NIAH-26 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DE 52 column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Aspergillus parasiticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus parasiticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000032 | - |
crude enzyme, in 60 mM potassium phosphate buffer (pH 7.5), at 30°C | Aspergillus parasiticus |
| 0.0222 | - |
after 694fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 30°C | Aspergillus parasiticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5'-hydroxyaverantin + NAD+ | - |
Aspergillus parasiticus | 5'-oxoaverantin + NADH + H+ | - |
? | |
| 5'-hydroxyaverantin + NAD+ | - |
Aspergillus parasiticus NIAH-26 | 5'-oxoaverantin + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 28000, SDS-PAGE | Aspergillus parasiticus |
| homodimer | 2 * 29600, calculated from amino acid sequence | Aspergillus parasiticus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AdhA | - |
Aspergillus parasiticus |
| HAVN dehydrogenase | - |
Aspergillus parasiticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Aspergillus parasiticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | - |
Aspergillus parasiticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Aspergillus parasiticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | required for activity | Aspergillus parasiticus | |
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