Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.351 extracted from

  • Stournaras, C.; Maurer, P.; Kurz, G.
    6-Phospho-D-gluconate dehydrogenase from Pseudomonas fluorescens. Properties and subunit structure (1983), Eur. J. Biochem., 130, 391-396.
    View publication on PubMed
Search for more literature for 1.1.1.351

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 4 * 31000, sedimentation equilibrium analysis Pseudomonas fluorescens
32000
-
 4 * 32000, SDS-PAGE Pseudomonas fluorescens
115000
-
 catalytically active form, gel filtration Pseudomonas fluorescens
126000
-
 native protein, gel filtration Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:D-glucose-grown cell
-
 Pseudomonas fluorescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.023
-
 using NAD+ as cosubstrate, in 50 mM Tris/HCl buffer pH 8.2, at 20°C Pseudomonas fluorescens
0.121
-
 using NADP+ as cosubstrate, in 50 mM Tris/HCl buffer pH 8.2, at 20°C Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
 Pseudomonas fluorescens D-ribulose 5-phosphate + CO2 + NADH + H+
-
 ?
6-phospho-D-gluconate + NADP+
-
 Pseudomonas fluorescens D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 32000, SDS-PAGE Pseudomonas fluorescens
homotetramer 4 * 31000, sedimentation equilibrium analysis Pseudomonas fluorescens

Synonyms

Synonyms Comment Organism
6-phospho-D-gluconate dehydrogenase (decarboxylating)
-
 Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme is active with both NAD+ and NADP+ Pseudomonas fluorescens
NADP+ the enzyme is active with both NAD+ and NADP+. The rate of reaction with NADP+ is 20%, of the rate with NAD+ Pseudomonas fluorescens

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas fluorescens isoelectric focusing
-
 4.6