Cloned (Comment) | Organism |
---|---|
gene F54C8.1, recombinant expression of C-terminally GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Caenorhabditis elegans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant detagged enzyme, hanging drop vapour diffusion method, 10 mg/ml protein in 25 mM sodium acetate trihydrate, pH 5.0, and 300 mM sodium chloride is mixed with an equal volume of reservoir solution, containing 21% PEG 3350, 0.2 M sodium chloride, 0.1 M MES, pH 6.5, for parallelepiped-shaped crystals and 23% PEG 3350, 0.2 M sodium chloride, 0.1 M bicine, pH 8.0, for cuboid-shaped crystals, equilibration against 0.20 ml reservoir solution, 3-5days at 18°C, X-ray diffraction structure determination and analysis of parallelepiped-shaped cuboid-shaped crystal at 1.60 A and 2.20 A resolution, respectively | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | - |
Caenorhabditis elegans | 5759 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ | Caenorhabditis elegans | - |
3-oxoacyl-CoA + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | P34439 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, cleavage of the GST-tag by PPase, followed by gel filtration, anion exchange chromatography, and ultrafiltration | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ | - |
Caenorhabditis elegans | 3-oxoacyl-CoA + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
F54C8.1 | - |
Caenorhabditis elegans |
HAD | - |
Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Caenorhabditis elegans | |
NADH | - |
Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
evolution | based on the chain length of the substrates, the HAD family is divided into three subclasses: 3-hydroxyacyl-CoA dehydrogenases (HADs), long-chain 3-hydroxyacyl-CoA dehydrogenases (LCHADs) and short-chain 3-hydroxyacyl-CoA dehydrogenases (SCHADs). HADs are soluble dimeric enzymes that exhibit substrate specificity for an acyl-chain length of C4-C10 and are previously referred to as short-chain HADs | Caenorhabditis elegans |
metabolism | 3-hydroxyacyl-CoA dehydrogenase catalyzes the third step in fatty acid beta-oxidation, oxidizing the hydroxyl group of 3-hydroxyacyl-CoA to a keto group | Caenorhabditis elegans |