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Literature summary for 1.1.1.35 extracted from

  • Liu, X.; Deng, G.; Chu, X.; Li, N.; Wu, L.; Li, D.
    Formation of an enolate intermediate is required for the reaction catalyzed by 3-hydroxyacyl-CoA dehydrogenase (2007), Bioorg. Med. Chem. Lett., 17, 3187-3190.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
N208A site-directed mutagenesis, the substrate binding of the mutant enzyme is affected Rattus norvegicus
S137A site-directed mutagenesis, the substrate binding of the mutant enzyme is affected Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of wild-type and mutant enzymes, overview Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxyacyl-CoA + NAD+ Rattus norvegicus
-
3-oxoacyl-CoA + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+ reaction mechanism via enolate intermediate Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxyacyl-CoA + NAD+
-
Rattus norvegicus 3-oxoacyl-CoA + NADH + H+
-
r
(S)-3-hydroxyacyl-CoA + NAD+ the enzyme is a B-side-specific dehydrogenase with hydride transfer occurring on the si face of the nicotinamide ring Rattus norvegicus 3-oxoacyl-CoA + NADH + H+
-
r
2-fluoro-3-hydroxy-4-octenoyl-CoA + NAD+
-
Rattus norvegicus 2-fluoro-3-oxo-4-octenoyl-CoA + NADH
-
r
2-fluoro-3-hydroxyoctanoyl-CoA + NAD+
-
Rattus norvegicus 2-fluoro-3-oxooctanoyl-CoA + NADH
-
r
3-hydroxy-4-octenoyl-CoA + NAD+
-
Rattus norvegicus 3-oxo-4-octenoyl-CoA + NADH
-
r
3-hydroxyoctanoyl-CoA + NAD+
-
Rattus norvegicus 3-oxooctanoyl-CoA + NADH
-
r
additional information no activity of the wild-type enzyme with 2,2-difluoro-3-hydroxyoctanoyl-CoA and 2,2-difluoro-3-hydroxy-4-octenoyl-CoA, substrate specificity of wild-type and mutant enzymes, y, overview Rattus norvegicus ?
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus