Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | NADPH has no inhibitory effect even at a concentration of 2.5 mM | Medicago sativa | |
vestitone | vestitone reductase is noticeably inhibited by vestitone concentrations in excess of 0.05 mM, the activity being inhibited over 40% at 0.25 mM vestitone | Medicago sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
(3R)-vestitone | in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C | Medicago sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Medicago sativa |
38000 | - |
1 * 38000, SDS-PAGE | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-vestitone + NADPH + H+ | Medicago sativa | vestitone reductase has strict substrate stereospecificity for (3R)-vestitone | (3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PEG fractionation, Red-agarose column chromatography, MonoQ column chromatography, and DEAE-Sephacel gel filtration | Medicago sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
crude extract, in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C | Medicago sativa |
461.1 | - |
after 1844fold purification, in 0.2 M sodium phosphate buffer, pH 6.0, at 30°C | Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-vestitone + NADPH + H+ | vestitone reductase has strict substrate stereospecificity for (3R)-vestitone | Medicago sativa | (3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Medicago sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Medicago sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Medicago sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Medicago sativa |