Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition at 0.5 mM | Corynebacterium sp. | |
Fe3+ | strong inhibition at 0.5 mM | Corynebacterium sp. | |
additional information | not inhibited by 2-dehydro-L-gulonate. There is no observed effect on activity by 0.5 mM solutions of Mg2+, Mn2+, Ca2+, or Co2+, 14 mM 2-mercaptoethanol, 1 mM dithiothreitol, or 1 mM EDTA | Corynebacterium sp. | |
NADP+ | competitive inhibition | Corynebacterium sp. | |
Ni2+ | strong inhibition at 0.5 mM | Corynebacterium sp. | |
Zn2+ | strong inhibition at 0.5 mM | Corynebacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | at pH 6.4 and 25°C | Corynebacterium sp. | |
0.125 | - |
NADP+ | at pH 9.2 and 25°C | Corynebacterium sp. | |
26 | - |
2,5-didehydro-D-gluconate | at pH 6.4 and 25°C | Corynebacterium sp. | |
155 | - |
5-dehydro-D-fructose | at pH 6.4 and 25°C | Corynebacterium sp. | |
160 | - |
dihydroxyacetone | at pH 6.4 and 25°C | Corynebacterium sp. | |
204 | - |
2-dehydro-L-gulonate | at pH 9.2 and 25°C | Corynebacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Corynebacterium sp. | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000, SDS-PAGE | Corynebacterium sp. |
35000 | - |
gel filtration | Corynebacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Corynebacterium sp. ATCC 31090 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, Cibacron blueF 3GA affinity column chromatography, and and TSK gel filtration | Corynebacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0052 | - |
crude extract, at pH 6.4 and 25°C | Corynebacterium sp. |
3.38 | - |
after 650fold purification, at pH 6.4 and 25°C | Corynebacterium sp. |
Storage Stability | Organism |
---|---|
-70°C, 2 mg/ml enzyme in 20 mM Tris-HC1 (pH 7.5), 6 months, the enzyme remains stable | Corynebacterium sp. |
4°C, enzyme solution at pH 6.5-7.5, at least 2 months, the enzyme remains stable | Corynebacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADPH + H+ | a reduction of substrate by NADPH is highly preferred | Corynebacterium sp. | 2-dehydro-L-gulonate + NADP+ | - |
r | |
2,5-didehydro-D-gluconate + NADPH + H+ | a reduction of substrate by NADPH is highly preferred | Corynebacterium sp. ATCC 31090 | 2-dehydro-L-gulonate + NADP+ | - |
r | |
2-dehydro-L-gulonate + NADP+ | - |
Corynebacterium sp. | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
r | |
5-dehydro-D-fructose + NADPH + H+ | - |
Corynebacterium sp. | ? + NADP+ | - |
? | |
dihydroxyacetone + NADPH + H+ | - |
Corynebacterium sp. | ? + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Corynebacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconate reductase | - |
Corynebacterium sp. |
2,5-DKG reductase | - |
Corynebacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
2,5-didehydro-D-gluconate | at pH 6.4 and 25°C | Corynebacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
for the reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
9.2 | - |
for the oxidation of 2-dehydro-L-gulonate | Corynebacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | for the reduction of 2,5-didehydro-D-gluconate | Corynebacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Corynebacterium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NADP+ | at pH 9.2 and 25°C | Corynebacterium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Corynebacterium sp. | isoelectric focusing | - |
4.4 |