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Literature summary for 1.1.1.343 extracted from

  • Pickl, A.; Schönheit, P.
    The oxidative pentose phosphate pathway in the haloarchaeon Haloferax volcanii involves a novel type of glucose-6-phosphate dehydrogenase - The archaeal Zwischenferment (2015), FEBS Lett., 589, 1108-1111.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
homologous overexpression of the his-tagged protein in Haloferax volcanii Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
6-phospho-D-gluconate pH 7.5, 42°C Haloferax volcanii
0.033
-
NAD+ pH 7.5, 42°C Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl highest activity at about 1 M KCl Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
4 * 37000, SDS-PAGE Haloferax volcanii
143000
-
gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NAD+ Haloferax volcanii the enzyme is essential for the biosynthesis of pentose phosphates from glucose-6-phosphate during growth of Haloferax volcanii on glucose as growth substrate D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+ Haloferax volcanii DSM 3757 the enzyme is essential for the biosynthesis of pentose phosphates from glucose-6-phosphate during growth of Haloferax volcanii on glucose as growth substrate D-ribulose 5-phosphate + CO2 + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GST8
-
-
Haloferax volcanii DSM 3757 D4GST8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD+
-
Haloferax volcanii D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+ the enzyme is essential for the biosynthesis of pentose phosphates from glucose-6-phosphate during growth of Haloferax volcanii on glucose as growth substrate Haloferax volcanii D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+
-
Haloferax volcanii DSM 3757 D-ribulose 5-phosphate + CO2 + NADH + H+
-
?
6-phospho-D-gluconate + NAD+ the enzyme is essential for the biosynthesis of pentose phosphates from glucose-6-phosphate during growth of Haloferax volcanii on glucose as growth substrate Haloferax volcanii DSM 3757 D-ribulose 5-phosphate + CO2 + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 37000, SDS-PAGE Haloferax volcanii

Synonyms

Synonyms Comment Organism
6PGDH ambiguous Haloferax volcanii
gndA
-
Haloferax volcanii
HVO_1830 locus name Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NAD+ the catalytic efficiency (kcat/Km) of the enzyme for NAD+ (174/s*mM) is about 2000fold higher than for NADP+ (0.082/s*mM) defining the enzyme as NAD+ specific Haloferax volcanii

General Information

General Information Comment Organism
malfunction compared to the wild type, the deletion mutant strain DELTAgndA does not grow, but growth is fully recovered by in-trans complementation with gndA. Growth of the deletion mutant can also be recovered by the addition of uridine to the medium, suggesting that Haloferax volcanii can circumvent the metabolic block for pentose phosphate formation via the oxidative pentose phosphate pathway by converting uridine to ribose-5-phosphate, catalyzed by uridine phosphorylase and phosphopentomutase Haloferax volcanii
physiological function the enzyme is essential for the biosynthesis of pentose phosphates from glucose-6-phosphate during growth of Haloferax volcanii on glucose as growth substrate Haloferax volcanii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
174
-
NAD+ pH 7.5, 42°C Haloferax volcanii