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Literature summary for 1.1.1.340 extracted from
- Pentalenolactone biosynthesis: Molecular cloning and assignment of biochemical function to PtlF, a short-chain dehydrogenase from Streptomyces avermitilis, and identification of a new biosynthetic intermediate (2007), Arch. Biochem. Biophys., 459, 233-240.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces avermitilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
1-deoxy-11beta-hydroxypentalenic acid | pH 8.0, 22°C | Streptomyces avermitilis |  |
| 0.025 | - |
NAD+ | pH 8.0, 22°C | Streptomyces avermitilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30183 | - |
x * 30183, MALDI-TOF, recombinant protein including His-tag | Streptomyces avermitilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | Q82IY9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-deoxy-11beta-hydroxypentalenic acid + NAD+ | - |
Streptomyces avermitilis | 1-deoxy-11-oxopentalenic acid + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30183, MALDI-TOF, recombinant protein including His-tag | Streptomyces avermitilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ptlF | - |
Streptomyces avermitilis |
| SAV_2933 | locus name | Streptomyces avermitilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
1-deoxy-11beta-hydroxypentalenic acid | pH 8.0, 22°C | Streptomyces avermitilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
and second maximum at pH 11.3 | Streptomyces avermitilis |
| 11.3 | - |
activity increases from pH 9.0 to pH 11.3, and is 12fold higher at pH 11.3 than at pH 8.0 | Streptomyces avermitilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | 150fold preference for NAD+ over NADP+ | Streptomyces avermitilis | |
| NADP+ | 150fold preference for NAD+ over NADP+ | Streptomyces avermitilis | |
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