Any feedback?
Literature summary for 1.1.1.340 extracted from
- Pentalenolactone biosynthesis: Molecular cloning and assignment of biochemical function to PtlF, a short-chain dehydrogenase from Streptomyces avermitilis, and identification of a new biosynthetic intermediate (2007), Arch. Biochem. Biophys., 459, 233-240.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces avermitilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
1-deoxy-11beta-hydroxypentalenic acid | pH 8.0, 22°C | Streptomyces avermitilis |  |
| 0.025 | - |
NAD+ | pH 8.0, 22°C | Streptomyces avermitilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30183 | - |
x * 30183, MALDI-TOF, recombinant protein including His-tag | Streptomyces avermitilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | Q82IY9 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-deoxy-11beta-hydroxypentalenic acid + NAD+ | - |
Streptomyces avermitilis | 1-deoxy-11-oxopentalenic acid + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30183, MALDI-TOF, recombinant protein including His-tag | Streptomyces avermitilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ptlF | - |
Streptomyces avermitilis |
| SAV_2933 | locus name | Streptomyces avermitilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
1-deoxy-11beta-hydroxypentalenic acid | pH 8.0, 22°C | Streptomyces avermitilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
and second maximum at pH 11.3 | Streptomyces avermitilis |
| 11.3 | - |
activity increases from pH 9.0 to pH 11.3, and is 12fold higher at pH 11.3 than at pH 8.0 | Streptomyces avermitilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | 150fold preference for NAD+ over NADP+ | Streptomyces avermitilis | |
| NADP+ | 150fold preference for NAD+ over NADP+ | Streptomyces avermitilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
