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Literature summary for 1.1.1.34 extracted from

  • Wei, H.; Xu, C.; Movahedi, A.; Sun, W.; Li, D.; Zhuge, Q.
    Characterization and function of 3-hydroxy-3-methylglutaryl-CoA reductase in Populus trichocarpa overexpression of PtHMGR enhances terpenoids in transgenic poplar (2019), Front. Plant Sci., 10, 1476 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.34

Cloned(Commentary)

Cloned (Comment) Organism
a truncated protein is expressed in Escherichia coli BL21(DE3) cells Populus trichocarpa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Populus trichocarpa
-
 (R)-mevalonate + CoA + 2 NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Populus trichocarpa B9GJZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Populus trichocarpa

Source Tissue

Source Tissue Comment Organism Textmining
leaf highest expression in young leaves Populus trichocarpa
-
petiole lowest expression Populus trichocarpa
-
root
-
 Populus trichocarpa
-
stem
-
 Populus trichocarpa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Populus trichocarpa (R)-mevalonate + CoA + 2 NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, truncated recombinant enzyme, SDS-PAGE Populus trichocarpa
? x * 61640, calculated from amino acid sequence Populus trichocarpa

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl-CoA reductase
-
 Populus trichocarpa
HMGR
-
 Populus trichocarpa

pI Value

Organism Comment pI Value Maximum pI Value
Populus trichocarpa calculated from amino acid sequence
-
 6.6

Expression

Organism Comment Expression
Populus trichocarpa enzyme expression is increased 6 h after treatment with 0.2 mM abscisic acid, 6 h after treatment with 200 mM NaCl, 3 days after treatment with 10% (w/v) PEG 6000, 1 h after treatment with 2 mM H2O2, and 7 days after cold treatment at 4°C up

General Information

General Information Comment Organism
metabolism enzyme overexpression significantly increases abscisic acid, gibberellic acid, carotene, and lycopene content, indicating that the enzyme participates in the regulation of terpenoid compound synthesis Populus trichocarpa