Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lovastatin | competitive inhibitor for HMG-CoA binding site | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
3-hydroxy-3-methylglutaryl-CoA | - |
Saccharolobus solfataricus | |
0.017 | - |
3-hydroxy-3-methylglutaryl-CoA | pH 5.5, 50°C | Saccharolobus solfataricus | |
0.023 | - |
NADPH | - |
Saccharolobus solfataricus | |
0.023 | - |
NADPH | pH 5.5, 50°C | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | Saccharolobus solfataricus | - |
(R)-mevalonate + CoA + NADP+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | Saccharolobus solfataricus P2 | - |
(R)-mevalonate + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q980N1 | - |
- |
Saccharolobus solfataricus P2 | Q980N1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
recombinant enzyme | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.5 | - |
50°C, pH 5.5, recombinant enzyme | Saccharolobus solfataricus |
17500 | - |
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Saccharolobus solfataricus | (R)-mevalonate + CoA + NADP+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | substrate is (S)-isomer of HMG-CoA | Saccharolobus solfataricus | (R)-mevalonate + CoA + NADP+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | - |
Saccharolobus solfataricus P2 | (R)-mevalonate + CoA + NADP+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + NADPH | substrate is (S)-isomer of HMG-CoA | Saccharolobus solfataricus P2 | (R)-mevalonate + CoA + NADP+ | - |
? | |
additional information | no substrate: NADH | Saccharolobus solfataricus | ? | - |
? | |
additional information | no substrate: NADH | Saccharolobus solfataricus P2 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
stable | Saccharolobus solfataricus |
90 | - |
half-life at 90°C is 3.2 h | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Saccharolobus solfataricus |