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Literature summary for 1.1.1.338 extracted from

  • Denger, K.; Cook, A.M.
    Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase (2010), Microbiology, 156, 967-974.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NADPH pH 9.0, 23°C Chromohalobacter salexigens
0.14
-
3-sulfopyruvate pH 9.0, 23°C Chromohalobacter salexigens
1.2
-
NADP+ pH 9.0, 23°C Chromohalobacter salexigens
19
-
(S)-3-sulfolactate pH 9.0, 23°C Chromohalobacter salexigens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Chromohalobacter salexigens

Organism

Organism UniProt Comment Textmining
Chromohalobacter salexigens Q1QWN5 DSM 3043
-

Storage Stability

Storage Stability Organism
-18°C, stable against freezing and thawing Chromohalobacter salexigens
4°C, stable for several weeks Chromohalobacter salexigens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-sulfolactate + NADP+ specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate Chromohalobacter salexigens 3-sulfopyruvate + NADPH + H+
-
r
3-sulfopyruvate + NADPH + H+ specific activity for oxidation of sulfolactate is about 5% of the activity for reduction of 3-sulfopyruvate Chromohalobacter salexigens (S)-3-sulfolactate + NADP+
-
r
additional information no substrate: pyruvate, oxaloacetate, (S)-malate, (S)-lactate, (R)-malate, (R)-lactate Chromohalobacter salexigens ?
-
?

Subunits

Subunits Comment Organism
? 2 * 37000, SDS-PAGE Chromohalobacter salexigens

Synonyms

Synonyms Comment Organism
ComC
-
Chromohalobacter salexigens

Cofactor

Cofactor Comment Organism Structure
additional information no cosubstrate: NADH Chromohalobacter salexigens
NADPH
-
Chromohalobacter salexigens

Expression

Organism Comment Expression
Chromohalobacter salexigens expression is induced by growth on sulfolactate up