Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfopyruvate | possible substrate inhibition at 0.60 mM | Methanobrevibacter millerae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | typical Michaelis-Menten kinetics, comparison to ComC enzymes from other rumen methanogens from Methanobrevibacter | Methanobrevibacter millerae | |
0.0551 | - |
NADH | pH 6.5, 37°C | Methanobrevibacter millerae | |
0.196 | - |
sulfopyruvate | pH 6.5, 37°C | Methanobrevibacter millerae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | required | Methanobrevibacter millerae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfopyruvate + NADH + H+ | Methanobrevibacter millerae | - |
sulfo-2-hydroxypropanoate + NAD+ | - |
r | |
sulfopyruvate + NADH + H+ | Methanobrevibacter millerae SM9 | - |
sulfo-2-hydroxypropanoate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobrevibacter millerae | A0A0U3EA38 | - |
- |
Methanobrevibacter millerae SM9 | A0A0U3EA38 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + H+ | 0.2% activity compared to sulfopyruvate | Methanobrevibacter millerae | 2-hydroxyglutarate + NAD+ | - |
r | |
2-oxoglutarate + NADH + H+ | 0.2% activity compared to sulfopyruvate | Methanobrevibacter millerae SM9 | 2-hydroxyglutarate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | 31% activity compared to sulfopyruvate | Methanobrevibacter millerae | malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | 31% activity compared to sulfopyruvate | Methanobrevibacter millerae SM9 | malate + NAD+ | - |
r | |
sulfopyruvate + NADH + H+ | - |
Methanobrevibacter millerae | sulfo-2-hydroxypropanoate + NAD+ | - |
r | |
sulfopyruvate + NADH + H+ | preferred substrate | Methanobrevibacter millerae | sulfo-2-hydroxypropanoate + NAD+ | - |
r | |
sulfopyruvate + NADH + H+ | - |
Methanobrevibacter millerae SM9 | sulfo-2-hydroxypropanoate + NAD+ | - |
r | |
sulfopyruvate + NADH + H+ | preferred substrate | Methanobrevibacter millerae SM9 | sulfo-2-hydroxypropanoate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
(R)-sulfolactate dehydrogenase | - |
Methanobrevibacter millerae |
ComC | - |
Methanobrevibacter millerae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanobrevibacter millerae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.9 | - |
NADH | pH 6.5, 37°C | Methanobrevibacter millerae | |
62.8 | - |
sulfopyruvate | pH 6.5, 37°C | Methanobrevibacter millerae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Methanobrevibacter millerae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | ComC shows less than 1% specific activity in the reduction reaction with NADPH compared to NADH. ComC which shows a 5fold lower KM for sulfopyruvate in the presence of NADH compared to NADPH (0.055 mM and 0.21 mM, resp.) and a 60fold higher Vmax/KM | Methanobrevibacter millerae | |
NAD+ | - |
Methanobrevibacter millerae | |
NADH | - |
Methanobrevibacter millerae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme ComC belongs to the LDH2/MDH2 oxidoreductase family | Methanobrevibacter millerae |
metabolism | the enzyme is involved in the coenzyme M biosynthesis pathway. Coenzyme M is required for methanogenesis to occur | Methanobrevibacter millerae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
320.4 | - |
sulfopyruvate | pH 6.5, 37°C | Methanobrevibacter millerae | |
887.5 | - |
NADH | pH 6.5, 37°C | Methanobrevibacter millerae |