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Literature summary for 1.1.1.335 extracted from
- Biochemical and structural characterization of WlbA from Bordetella pertussis and Chromobacterium violaceum: Enzymes required for the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid (2011), Biochemistry, 50, 1483-1491.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bordetella pertussis |
| expression in Escherichia coli | Chromobacterium violaceum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in presence of NAD(H) and substrate to 2.13 A and 1.5 A resolution. Enzyme displays octameric quaternary structure with the active sites positioned far apart. The octamers can be envisioned as tetramers of dimers. The carboxylate group attached to the C-5' carbon of the hexose in the natural substrate, UDP-N-acetyl-D-glucosaminuronic acid, is held firmly in place in the enzyme WlbA active site by the side chains of Arg165 and Tyr169 | Bordetella pertussis |
| in presence of NAD(H) and substrate to 2.13 A and 1.5 A resolution. Enzyme displays octameric quaternary structure with the active sites positioned far apart. The octamers can be envisioned as tetramers of dimers. The carboxylate group attached to the C-5' carbon of the hexose in the natural substrate, UDP-N-acetyl-D-glucosaminuronic acid, is held firmly in place in the enzyme WlbA active site by the side chains of Arg165 and Tyr169 | Chromobacterium violaceum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0056 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Chromobacterium violaceum |  |
| 0.015 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Bordetella pertussis | |
| 0.2 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.47 | - |
NAD+ | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.97 | - |
NAD+ | 25°C, pH 8.5 | Bordetella pertussis | |
| 1.1 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 2.05 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
| 2.36 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bordetella pertussis | - |
- |
- |
| Chromobacterium violaceum | Q7NQL0 | putative | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the reaction mechanism is sequential, enzyme does not require 2-ketoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine | Bordetella pertussis | ? | - |
? | |
| additional information | the reaction mechanism is sequential, enzyme does not require 2-oxoglutarate for activity. No substrates: UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine | Chromobacterium violaceum | ? | - |
? | |
| UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ | - |
Bordetella pertussis | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
| UDP-2-acetamido-2-deoxy-alpha-D-glucuronate + NAD+ | - |
Chromobacterium violaceum | UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
| UDP-alpha-D-glucuronate + NAD+ | - |
Bordetella pertussis | UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
| UDP-alpha-D-glucuronate + NAD+ | - |
Chromobacterium violaceum | UDP-alpha-D-ribo-hex-3-uluronate + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| WlbA | - |
Bordetella pertussis |
| WlbA | - |
Chromobacterium violaceum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00015 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.00031 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.00076 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.0021 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
| 0.0039 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Bordetella pertussis | |
| 0.0047 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bordetella pertussis | |
| NAD+ | - |
Chromobacterium violaceum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00028 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.00075 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.001 | - |
UDP-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
| 0.002 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | 25°C, pH 8.5 | Bordetella pertussis | |
| 0.136 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Chromobacterium violaceum | |
| 0.26 | - |
UDP-2-acetamido-2-deoxy-alpha-D-glucuronate | presence of transaminase WlbC, 25°C, pH 8.5 | Bordetella pertussis | |
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