Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trichophyton benhamiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM, 71% of initial activity | Trichophyton benhamiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
chanoclavine-I | pH 7.5, 37°C | Trichophyton benhamiae | |
0.36 | - |
NAD+ | pH 7.5, 37°C | Trichophyton benhamiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, addition to EDTA-treated enzyme, 31% of control activity | Trichophyton benhamiae | |
Mn2+ | 5 mM, addition to EDTA-treated enzyme, 35% of control activity | Trichophyton benhamiae | |
Ni2+ | 5 mM, addition to EDTA-treated enzyme, 73% of control activity | Trichophyton benhamiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26200 | - |
4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein | Trichophyton benhamiae |
30000 | - |
4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein | Trichophyton benhamiae |
128000 | - |
gel filtration | Trichophyton benhamiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichophyton benhamiae | D4AK45 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chanoclavine-I + NAD+ | - |
Trichophyton benhamiae | chanoclavine-I aldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 26200, calculated, 4 * 30000, SDS-PAGE of recombinant His-tagged protein | Trichophyton benhamiae |
Synonyms | Comment | Organism |
---|---|---|
ARB_04646 | - |
Trichophyton benhamiae |
ChaDH | - |
Trichophyton benhamiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
chanoclavine-I | pH 7.5, 37°C | Trichophyton benhamiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+, FMN or FAD | Trichophyton benhamiae | |
NAD+ | specific for NAD+ | Trichophyton benhamiae |