An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism

Mihasan, M.; Chiribau, C.B.; Friedrich, T.; Artenie, V.; Brandsch, R.; Appl. Environ. Microbiol. 73, 2479-2485 (2007)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.0062

-

1,4-Naphthoquinone

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

0.0088

-

1,4-benzoquinone

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

0.023

-

oxidized 2,6-dichlorophenol-indophenol

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

0.035

-

NADH

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

1,4-benzoquinone + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

1,4-benzoquinol + NADP+

-

-

-

?

1,4-naphthoquinone + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

1,4-naphthoquinol + NADP+

-

-

-

?

4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

? + NADP+

-

-

-

?

duroquinone + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

duroquinol + NADP+

-

-

-

?

menadione + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

menadiol + NADP+

-

-

-

?

nicotine blue + NADH + H+

-

718625

Paenarthrobacter nicotinovorans

nicotine blue leuco form + NAD+

two-electron transfer reaction

-

-

?

nicotine blue + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

nicotine blue leuco form + NADP+

two-electron transfer reaction

-

-

?

oxidized 2,6-dichlorophenol-indophenol + NADH + H+

-

718625

Paenarthrobacter nicotinovorans

reduced 2,6-dichlorophenol-indophenol + NAD+

-

-

-

r

oxidized 2,6-dichlorophenol-indophenol + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

reduced 2,6-dichlorophenol-indophenol + NADP+

-

-

-

?

Cofactor

Commentary

Organism

Structure

FMN

-

Paenarthrobacter nicotinovorans

NADH

NADH is prefered over NADPH

Paenarthrobacter nicotinovorans

NADPH

70% of the rate with NADH

Paenarthrobacter nicotinovorans

Cofactor

Commentary

Organism

Structure

FMN

-

Paenarthrobacter nicotinovorans

NADH

NADH is prefered over NADPH

Paenarthrobacter nicotinovorans

NADPH

70% of the rate with NADH

Paenarthrobacter nicotinovorans

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.0062

-

1,4-Naphthoquinone

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

0.0088

-

1,4-benzoquinone

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

0.023

-

oxidized 2,6-dichlorophenol-indophenol

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

0.035

-

NADH

pH 7.1, temperature not specified in the publication

Paenarthrobacter nicotinovorans

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

1,4-benzoquinone + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

1,4-benzoquinol + NADP+

-

-

-

?

1,4-naphthoquinone + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

1,4-naphthoquinol + NADP+

-

-

-

?

4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

? + NADP+

-

-

-

?

duroquinone + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

duroquinol + NADP+

-

-

-

?

menadione + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

menadiol + NADP+

-

-

-

?

nicotine blue + NADH + H+

-

718625

Paenarthrobacter nicotinovorans

nicotine blue leuco form + NAD+

two-electron transfer reaction

-

-

?

nicotine blue + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

nicotine blue leuco form + NADP+

two-electron transfer reaction

-

-

?

oxidized 2,6-dichlorophenol-indophenol + NADH + H+

-

718625

Paenarthrobacter nicotinovorans

reduced 2,6-dichlorophenol-indophenol + NAD+

-

-

-

r

oxidized 2,6-dichlorophenol-indophenol + NADPH + H+

-

718625

Paenarthrobacter nicotinovorans

reduced 2,6-dichlorophenol-indophenol + NADP+

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

718625

Mihasan

An NAD(P)H-nicotine blue oxido ...

Paenarthrobacter nicotinovorans

Appl. Environ. Microbiol.

73

2479-2485

2007

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