Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli
Paenarthrobacter nicotinovorans
KM Value [mM]
0.0062
1,4-Naphthoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.0088
1,4-benzoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.023
oxidized 2,6-dichlorophenol-indophenol
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.035
NADH
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Organism
Paenarthrobacter nicotinovorans
Specific Activity [micromol/min/mg]
0.004
substrate duraquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.102
substrate menadione, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.368
substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.536
substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.733
substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.893
substrate nicotine blue, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate)
1,4-benzoquinone + NADPH + H+
718625
Paenarthrobacter nicotinovorans
1,4-benzoquinol + NADP+
?
1,4-naphthoquinone + NADPH + H+
718625
Paenarthrobacter nicotinovorans
1,4-naphthoquinol + NADP+
?
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
718625
Paenarthrobacter nicotinovorans
? + NADP+
?
duroquinone + NADPH + H+
718625
Paenarthrobacter nicotinovorans
duroquinol + NADP+
?
menadione + NADPH + H+
718625
Paenarthrobacter nicotinovorans
menadiol + NADP+
?
nicotine blue + NADH + H+
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NAD+
two-electron transfer reaction
?
nicotine blue + NADPH + H+
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NADP+
two-electron transfer reaction
?
oxidized 2,6-dichlorophenol-indophenol + NADH + H+
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NAD+
r
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NADP+
?
Synonyms
NAD(P)H-nicotine blue oxidoreductase
Paenarthrobacter nicotinovorans
NAD(P)H-nicotine blue quinone oxidoreductase
Paenarthrobacter nicotinovorans
nboR
Paenarthrobacter nicotinovorans
Cofactor
FMN
Paenarthrobacter nicotinovorans
NADH
NADH is prefered over NADPH
Paenarthrobacter nicotinovorans
NADPH
70% of the rate with NADH
Paenarthrobacter nicotinovorans
Cloned(Commentary) (protein specific)
expression in Escherichia coli
Paenarthrobacter nicotinovorans
Cofactor (protein specific)
FMN
Paenarthrobacter nicotinovorans
NADH
NADH is prefered over NADPH
Paenarthrobacter nicotinovorans
NADPH
70% of the rate with NADH
Paenarthrobacter nicotinovorans
KM Value [mM] (protein specific)
0.0062
1,4-Naphthoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.0088
1,4-benzoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.023
oxidized 2,6-dichlorophenol-indophenol
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.035
NADH
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Specific Activity [micromol/min/mg] (protein specific)
0.004
substrate duraquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.102
substrate menadione, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.368
substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.536
substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.733
substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.893
substrate nicotine blue, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate) (protein specific)
1,4-benzoquinone + NADPH + H+
718625
Paenarthrobacter nicotinovorans
1,4-benzoquinol + NADP+
?
1,4-naphthoquinone + NADPH + H+
718625
Paenarthrobacter nicotinovorans
1,4-naphthoquinol + NADP+
?
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
718625
Paenarthrobacter nicotinovorans
? + NADP+
?
duroquinone + NADPH + H+
718625
Paenarthrobacter nicotinovorans
duroquinol + NADP+
?
menadione + NADPH + H+
718625
Paenarthrobacter nicotinovorans
menadiol + NADP+
?
nicotine blue + NADH + H+
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NAD+
two-electron transfer reaction
?
nicotine blue + NADPH + H+
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NADP+
two-electron transfer reaction
?
oxidized 2,6-dichlorophenol-indophenol + NADH + H+
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NAD+
r
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NADP+
?
Expression
Paenarthrobacter nicotinovorans
enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR
additional information
General Information
physiological function
enzyme is part of the nicotine catabolic pathway
Paenarthrobacter nicotinovorans
General Information (protein specific)
physiological function
enzyme is part of the nicotine catabolic pathway
Paenarthrobacter nicotinovorans
Expression (protein specific)
Paenarthrobacter nicotinovorans
enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR
additional information
Other publictions for EC 1.1.1.328
718625
Mihasan
An NAD(P)H-nicotine blue oxido ...
Paenarthrobacter nicotinovorans
Appl. Environ. Microbiol.
73
2479-2485
2007
-
-
1
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
6
-
9
-
3
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
6
-
9
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-