BRENDA - Enzyme Database
show all sequences of 1.1.1.328

An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism

Mihasan, M.; Chiribau, C.B.; Friedrich, T.; Artenie, V.; Brandsch, R.; Appl. Environ. Microbiol. 73, 2479-2485 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Paenarthrobacter nicotinovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
1,4-Naphthoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.0088
-
1,4-benzoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.023
-
oxidized 2,6-dichlorophenol-indophenol
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.035
-
NADH
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Organism
Organism
UniProt
Commentary
Textmining
Paenarthrobacter nicotinovorans
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.004
-
substrate duraquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.102
-
substrate menadione, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.368
-
substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.536
-
substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.733
-
substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.893
-
substrate nicotine blue, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,4-benzoquinone + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
1,4-benzoquinol + NADP+
-
-
-
?
1,4-naphthoquinone + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
1,4-naphthoquinol + NADP+
-
-
-
?
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
? + NADP+
-
-
-
?
duroquinone + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
duroquinol + NADP+
-
-
-
?
menadione + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
menadiol + NADP+
-
-
-
?
nicotine blue + NADH + H+
-
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NAD+
two-electron transfer reaction
-
-
?
nicotine blue + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NADP+
two-electron transfer reaction
-
-
?
oxidized 2,6-dichlorophenol-indophenol + NADH + H+
-
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NAD+
-
-
-
r
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
NAD(P)H-nicotine blue oxidoreductase
-
Paenarthrobacter nicotinovorans
NAD(P)H-nicotine blue quinone oxidoreductase
-
Paenarthrobacter nicotinovorans
nboR
-
Paenarthrobacter nicotinovorans
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
-
Paenarthrobacter nicotinovorans
NADH
NADH is prefered over NADPH
Paenarthrobacter nicotinovorans
NADPH
70% of the rate with NADH
Paenarthrobacter nicotinovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Paenarthrobacter nicotinovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
-
Paenarthrobacter nicotinovorans
NADH
NADH is prefered over NADPH
Paenarthrobacter nicotinovorans
NADPH
70% of the rate with NADH
Paenarthrobacter nicotinovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
1,4-Naphthoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.0088
-
1,4-benzoquinone
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.023
-
oxidized 2,6-dichlorophenol-indophenol
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.035
-
NADH
pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.004
-
substrate duraquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.102
-
substrate menadione, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.368
-
substrate 2,6-dichlorophenol-indophenol, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.536
-
substrate 1,4-benzoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.733
-
substrate 1,4-naphthoquinone, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
0.893
-
substrate nicotine blue, pH 7.1, temperature not specified in the publication
Paenarthrobacter nicotinovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,4-benzoquinone + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
1,4-benzoquinol + NADP+
-
-
-
?
1,4-naphthoquinone + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
1,4-naphthoquinol + NADP+
-
-
-
?
4,4',5,5'-tetrahydroxy-3,3'-diazadiphenoquinone-(2,2') + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
? + NADP+
-
-
-
?
duroquinone + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
duroquinol + NADP+
-
-
-
?
menadione + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
menadiol + NADP+
-
-
-
?
nicotine blue + NADH + H+
-
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NAD+
two-electron transfer reaction
-
-
?
nicotine blue + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
nicotine blue leuco form + NADP+
two-electron transfer reaction
-
-
?
oxidized 2,6-dichlorophenol-indophenol + NADH + H+
-
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NAD+
-
-
-
r
oxidized 2,6-dichlorophenol-indophenol + NADPH + H+
-
718625
Paenarthrobacter nicotinovorans
reduced 2,6-dichlorophenol-indophenol + NADP+
-
-
-
?
Expression
Organism
Commentary
Expression
Paenarthrobacter nicotinovorans
enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR
additional information
General Information
General Information
Commentary
Organism
physiological function
enzyme is part of the nicotine catabolic pathway
Paenarthrobacter nicotinovorans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme is part of the nicotine catabolic pathway
Paenarthrobacter nicotinovorans
Expression (protein specific)
Organism
Commentary
Expression
Paenarthrobacter nicotinovorans
enzyme gene is expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR
additional information
Other publictions for EC 1.1.1.328
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718625
Mihasan
An NAD(P)H-nicotine blue oxido ...
Paenarthrobacter nicotinovorans
Appl. Environ. Microbiol.
73
2479-2485
2007
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