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Literature summary for 1.1.1.327 extracted from

  • Hofer, M.; Strittmatter, H.; Sieber, V.
    Biocatalytic synthesis of a diketobornane as a building block for bifunctional camphor derivatives (2013), ChemCatChem, 5, 3351-3357.
No PubMed abstract available
Search for more literature for 1.1.1.327

Application

Application Comment Organism
synthesis synthetic route for the synthesis of bifunctional camphor derivatives. The combination of the enzymatic P450cam system with 5-exo-hydroxycamphor dehydrogenase (FdeH) allows an efficient synthesis of 2,5-diketobornane, which can be used for further derivatisation Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0279
-
 5-exo-hydroxycamphor pH 8.0, 45°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P09347 sequence shows one nucleotide exchange at position 336, at which replacement of a guanine by a cytosine takes place
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-exo-hydroxycamphor + NAD+
-
 Pseudomonas putida bornane-2,5-dione + NADH + H+
-
 r
bornane-2,5-dione + NADH + H+
-
 Pseudomonas putida 5-exo-hydroxycamphor + NAD+
-
 r
additional information no substrate: camphor Pseudomonas putida ?
-
 ?

Synonyms

Synonyms Comment Organism
camD
-
 Pseudomonas putida
FdeH
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 no residual activity Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pseudomonas putida