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Literature summary for 1.1.1.323 extracted from

  • Croteau, R.; Felton, N.
    Substrate specificity of monoterpenol dehydrogenases from Foeniculum vulgare and Tanacetum vulgare (1980), Phytochemistry, 19, 1343-1347.
No PubMed abstract available
Search for more literature for 1.1.1.323

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 (+)-thujan-3-ol pH 8.0, 30°C Tanacetum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66500
-
 gel filtration Tanacetum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-thujan-3-ol + NAD(P)+ Tanacetum vulgare
-
 (+)-thujan-3-one + NAD(P)H + H+
-
 ?
additional information Tanacetum vulgare leaves of Tanacetum vulgare catalyse the pyridine nucleotide-dependent dehydrogenation of D-3-thujanol to D-3-thujone ?
-
 ?

Organism

Organism UniProt Comment Textmining
Tanacetum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by gel filtration Tanacetum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Tanacetum vulgare
-

Storage Stability

Storage Stability Organism
the partially purified enzyme is most stable in 50 mM sodium phosphate buffer, pH 8.0 Tanacetum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-thujan-3-ol + NAD(P)+
-
 Tanacetum vulgare (+)-thujan-3-one + NAD(P)H + H+
-
 ?
additional information leaves of Tanacetum vulgare catalyse the pyridine nucleotide-dependent dehydrogenation of D-3-thujanol to D-3-thujone Tanacetum vulgare ?
-
 ?

Synonyms

Synonyms Comment Organism
TDH
-
 Tanacetum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Tanacetum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Tanacetum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
6.7 9.3 50% of maximal activity at pH 6.7 and pH 9.3 Tanacetum vulgare

Cofactor

Cofactor Comment Organism Structure
NAD(P)+ TDH shows a slight preference for NAD+ over NADP+ as cofactor Tanacetum vulgare