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Literature summary for extracted from

  • Wu, Z.; Yang, Y.; Shaw, N.; Bhattacharya, S.; Yan, L.; West, K.; Roth, K.; Noy, N.; Qin, J.; Crabb, J.W.
    Mapping the ligand binding pocket in the cellular retinaldehyde binding protein (2003), J. Biol. Chem., 278, 12390-12396.
    View publication on PubMed


Cloned (Comment) Organism
expression in Hi-5 insect cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11-cis-retinol-[cellular retinaldehyde binding protein] wild-type cellular retinaldehyde binding protein, recombinant wild-type RDH5, pH 7.5, temperature not specified in the publication Homo sapiens


Organism UniProt Comment Textmining
Homo sapiens Q92781

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-cis-retinol-[cellular retinaldehyde binding protein] + NAD+ cellular retinaldehyde binding protein CRALBP serves as an 11-cis-retinol acceptor for the enzymatic isomerization of all-trans- to 11-cis-retinol and as a substrate carrier for 11-cis-retinol dehydrogenase RDH5. Altered kinetic parameters are observed for RDH5 oxidation of 11-cis-retinol bound to rCRALBP mutants M222A, M225A, and W244F, supporting impaired substrate carrier function. Data implicate Trp165, Met208, Met222, Met225, and Trp244 as components of the CRALBP ligand binding cavity Homo sapiens 11-cis-retinal-[cellular retinaldehyde binding protein] + NADH + H+