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Literature summary for 1.1.1.313 extracted from

  • Krejcík, Z.; Hollemeyer, K.; Smits, T.H.; Cook, A.M.
    Isethionate formation from taurine in Chromohalobacter salexigens: purification of sulfoacetaldehyde reductase (2010), Microbiology, 156, 1547-1555.
    View publication on PubMed
Search for more literature for 1.1.1.313

Inhibitors

Inhibitors Comment Organism Structure
4-oxobutyrate 90% substrate inhibition at 20 mM Chromohalobacter israelensis
additional information not inhibited by formaldehyde, acetaldehyde, betaine aldehyde, propionaldehyde, DL-glyceraldehyde, phosphonoacetaldehyde, glyoxylate, 2-oxobutyrate, 4-oxobutyrate and 3-sulfopropanaldehyde Chromohalobacter israelensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 NADPH in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C Chromohalobacter israelensis
0.13
-
 2-sulfoacetaldehyde in 0.02 mMTris/H2SO4 buffer, pH 9.0, at 23°C Chromohalobacter israelensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 SDS-PAGE Chromohalobacter israelensis
27100
-
 estimated from amino acid sequence Chromohalobacter israelensis

Organism

Organism UniProt Comment Textmining
Chromohalobacter israelensis
-
-
-
Chromohalobacter israelensis DSM 3043
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography, phenyl Superose gel filtration Chromohalobacter israelensis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ammonium-grown cell IsfD is detected at low levels in extracts of ammonium-grown cells Chromohalobacter israelensis
-
culture condition:taurine-grown cell IsfD is detected at high levels in extracts of taurine-grown cells Chromohalobacter israelensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter israelensis isethionate + NADP+
-
 ir
2-sulfoacetaldehyde + NADPH + H+
-
 Chromohalobacter israelensis DSM 3043 isethionate + NADP+
-
 ir
4-oxobutyrate + NADPH + H+
-
 Chromohalobacter israelensis ?
-
 ir
4-oxobutyrate + NADPH + H+
-
 Chromohalobacter israelensis DSM 3043 ?
-
 ir
additional information NADH is not a substrate Chromohalobacter israelensis ?
-
 ?
additional information NADH is not a substrate Chromohalobacter israelensis DSM 3043 ?
-
 ?

Synonyms

Synonyms Comment Organism
isethionate formation, reductase D
-
 Chromohalobacter israelensis
IsfD isoform, this enzyme represents the major portion of the IsfD activity described for taurine-grown cells Chromohalobacter israelensis
IsfD2 isoform, this enzyme is responsible for the low activity observed in extracts of ammonium-grown cells Chromohalobacter israelensis
NADPH-dependent sulfoacetaldehyde reductase
-
 Chromohalobacter israelensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 in Tris/H2SO4 buffer Chromohalobacter israelensis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 IsfD shows a broad pH optimum from about pH 6.5 to pH 9.5. There is a steep increase in activity from pH 4.5 (negligible activity) and an equally steep decrease above pH 9.5 Chromohalobacter israelensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
 IsfD is stable in Tris/H2SO4 buffer, pH 9.0 Chromohalobacter israelensis

Cofactor

Cofactor Comment Organism Structure
NADPH highly specific for NADPH. NADH is not a substrate. Chromohalobacter israelensis