Application | Comment | Organism |
---|---|---|
synthesis | the enzyme recombinantly expressed in Escherichia coli is used for chemoenzymatic synthesis of enantiomerically enriched (S)- and (R)-gamma-aryl-gamma-butyrolactones, whereby the key step is an enzyme-catalyzed stereoselective reduction of methyl 4-oxo-4-arylbutanoates, (S)-PED-catalyzed bioreduction in preparative scale | Aromatoleum aromaticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | Aromatoleum aromaticum | - |
acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | Aromatoleum aromaticum EbN1 | - |
acetophenone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aromatoleum aromaticum | Q5P5I4 | - |
- |
Aromatoleum aromaticum EbN1 | Q5P5I4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | - |
Aromatoleum aromaticum | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | - |
Aromatoleum aromaticum EbN1 | acetophenone + NADH + H+ | - |
r | |
acetophenone + NADH + H+ | - |
Aromatoleum aromaticum | (S)-1-phenylethanol + NAD+ | - |
r | |
acetophenone + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-1-phenylethanol + NAD+ | - |
r | |
methyl 4-(4-bromophenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-(4-bromophenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-bromophenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-(4-bromophenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-methyl 4-(4-bromophenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-bromophenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-(4-chlorophenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-(4-chlorophenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-chlorophenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-(4-chlorophenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum EbN1 | (S)-methyl 4-(4-chlorophenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-chlorophenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-(4-fluorophenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-(4-fluorophenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-fluorophenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-(4-methoxyphenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-(4-methoxyphenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-methoxyphenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-(4-methylphenyl)-4-oxobutanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-(4-methylphenyl)-4-hydroxybutanoate + (S)-(-)-5-(4-methylphenyl)dihydrofuran-2(3H)-one + NAD+ | - |
r | |
methyl 4-oxo-4-(pyren-1-yl)butanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-hydroxy-4-(pyren-1-yl)butanoate + (S)-(-)-5-(pyren-1-yl)oxolan-2-one + NAD+ | - |
r | |
methyl 4-oxo-4-phenylbutanoate + NADH + H+ | - |
Aromatoleum aromaticum | (S)-methyl 4-hydroxy-4-phenylbutanoate + (S)-(-)-5-phenyldihydrofuran-2(3H)-one + NAD+ | - |
r | |
additional information | the enzyme is used for chemoenzymatic synthesis of enantiomerically enriched (S)- and (R)-gamma-aryl-gamma-butyrolactones, whereby the key step is an enzyme-catalyzed stereoselective reduction of methyl 4-oxo-4-arylbutanoates, (S)-PED-catalyzed bioreduction | Aromatoleum aromaticum | ? | - |
- |
|
additional information | the enzyme is used for chemoenzymatic synthesis of enantiomerically enriched (S)- and (R)-gamma-aryl-gamma-butyrolactones, whereby the key step is an enzyme-catalyzed stereoselective reduction of methyl 4-oxo-4-arylbutanoates, (S)-PED-catalyzed bioreduction | Aromatoleum aromaticum EbN1 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
(S)-PED | - |
Aromatoleum aromaticum |
PED | - |
Aromatoleum aromaticum |
prelog-specific NADH-dependent (S)-1-phenylethanol dehydrogenase | - |
Aromatoleum aromaticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aromatoleum aromaticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aromatoleum aromaticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aromatoleum aromaticum | |
NADH | - |
Aromatoleum aromaticum |