BRENDA - Enzyme Database show
show all sequences of 1.1.1.310

Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase

Denger, K.; Cook, A.M.; Microbiology 156, 967-974 (2010)

Data extracted from this reference:

General Stability
General Stability
Organism
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
Chromohalobacter salexigens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate
Chromohalobacter salexigens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NADH
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
0.14
-
3-sulfopyruvate
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
2
-
NAD+
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
7
-
(2S)-3-sulfolactate
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 34000, SDS-PAGE
Chromohalobacter salexigens
61000
-
native enzyme, gel filtration
Chromohalobacter salexigens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Chromohalobacter salexigens
-
-
-
Chromohalobacter salexigens DSM 3043
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration
Chromohalobacter salexigens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:acetate-grown cell
-
Chromohalobacter salexigens
-
culture condition:sulfolactate-grown cell
-
Chromohalobacter salexigens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.2
-
crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
Chromohalobacter salexigens
7.4
-
crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
Chromohalobacter salexigens
675
-
enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
Chromohalobacter salexigens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-3-sulfolactate + NAD+
the specific activity is only about 5% of that in the reverse direction
705586
Chromohalobacter salexigens
3-sulfopyruvate + NADH + H+
-
-
-
r
(2S)-3-sulfolactate + NAD+
the specific activity is only about 5% of that in the reverse direction
705586
Chromohalobacter salexigens DSM 3043
3-sulfopyruvate + NADH + H+
-
-
-
r
3-sulfopyruvate + NADH + H+
-
705586
Chromohalobacter salexigens
(2S)-3-sulfolactate + NAD+
-
-
-
r
3-sulfopyruvate + NADH + H+
-
705586
Chromohalobacter salexigens DSM 3043
(2S)-3-sulfolactate + NAD+
-
-
-
r
additional information
neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate
705586
Chromohalobacter salexigens
?
-
-
-
-
additional information
neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate
705586
Chromohalobacter salexigens DSM 3043
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 34000, SDS-PAGE
Chromohalobacter salexigens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
Chromohalobacter salexigens
General Stability (protein specific)
General Stability
Organism
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
Chromohalobacter salexigens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate
Chromohalobacter salexigens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NADH
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
0.14
-
3-sulfopyruvate
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
2
-
NAD+
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
7
-
(2S)-3-sulfolactate
apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C
Chromohalobacter salexigens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 34000, SDS-PAGE
Chromohalobacter salexigens
61000
-
native enzyme, gel filtration
Chromohalobacter salexigens
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration
Chromohalobacter salexigens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:acetate-grown cell
-
Chromohalobacter salexigens
-
culture condition:sulfolactate-grown cell
-
Chromohalobacter salexigens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.2
-
crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
Chromohalobacter salexigens
7.4
-
crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
Chromohalobacter salexigens
675
-
enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C
Chromohalobacter salexigens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-3-sulfolactate + NAD+
the specific activity is only about 5% of that in the reverse direction
705586
Chromohalobacter salexigens
3-sulfopyruvate + NADH + H+
-
-
-
r
(2S)-3-sulfolactate + NAD+
the specific activity is only about 5% of that in the reverse direction
705586
Chromohalobacter salexigens DSM 3043
3-sulfopyruvate + NADH + H+
-
-
-
r
3-sulfopyruvate + NADH + H+
-
705586
Chromohalobacter salexigens
(2S)-3-sulfolactate + NAD+
-
-
-
r
3-sulfopyruvate + NADH + H+
-
705586
Chromohalobacter salexigens DSM 3043
(2S)-3-sulfolactate + NAD+
-
-
-
r
additional information
neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate
705586
Chromohalobacter salexigens
?
-
-
-
-
additional information
neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate
705586
Chromohalobacter salexigens DSM 3043
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 34000, SDS-PAGE
Chromohalobacter salexigens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
-
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity
Chromohalobacter salexigens
Other publictions for EC 1.1.1.310
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
705586
Denger
Racemase activity effected by ...
Chromohalobacter salexigens, Chromohalobacter salexigens DSM 3043
Microbiology
156
967-974
2010
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1
1
4
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-
2
-
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6
-
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1
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2
3
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6
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
4
-
-
2
-
-
-
-
1
-
2
3
-
6
1
-
-
1
-
-
-
-
-
-
-
-
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