Any feedback?
Literature summary for 1.1.1.31 extracted from
- 3-Hydroxyisobutyrate dehydrogenase is involved in both, valine and isoleucine degradation in Arabidopsis thaliana (2017), Plant Physiol., 175, 51-61 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
NAD+ | 25°C, pH 8.5 | Arabidopsis thaliana |  |
| 0.35 | - |
NAD+ | at pH 8.5 and 25°C | Arabidopsis thaliana | |
| 0.687 | - |
3-hydroxy-2-methylpropanoate | 25°C, pH 8.5 | Arabidopsis thaliana | |
| 0.687 | - |
(S)-3-hydroxyisobutyrate | at pH 8.5 and 25°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37400 | - |
calculated from sequence | Arabidopsis thaliana |
| 150000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-hydroxyisobutyrate + NAD+ | Arabidopsis thaliana | - |
2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
| 3-hydroxy-2-methylpropanoate + NAD+ | Arabidopsis thaliana | - |
2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Arabidopsis thaliana | Q9SUC0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxyisobutyrate + NAD+ | 2% activity compared to (S)-3-hydroxyisobutyrate | Arabidopsis thaliana | 2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
| (S)-3-hydroxyisobutyrate + NAD+ | - |
Arabidopsis thaliana | 2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
| (S)-3-hydroxyisobutyrate + NAD+ | 100% activity | Arabidopsis thaliana | methylmalonate semialdehyde + NADH + H+ | - |
? | |
| (S)-methyl-3-hydroxy-2-methylpropionate + NAD+ | 1.2% activity compared to (S)-3-hydroxyisobutyrate | Arabidopsis thaliana | ? | - |
? | |
| 3-hydroxy-2-methylpropanoate + NAD+ | - |
Arabidopsis thaliana | 2-methyl-3-oxopropanoate + NADH + H+ | - |
? | |
| 3-hydroxypropionate + NAD+ | 13% activity compared to (S)-3-hydroxyisobutyrate | Arabidopsis thaliana | malonate semialdehyde + NADH + H+ | - |
? | |
| additional information | no activity with (R)-methyl-3-hydroxy-2-methylpropionate, glycerate, serine, beta-hydroxypyruvate, threonine, 2-hydroxybutyrate, malate, glycolate, beta-hydroxybutyrate, lactate, and 6-phosphogluconate. NADP+ is not a suitable cofactor. Also, FAD, oxidized cytochrome c, as well as the artificial electron acceptors phenazine methosulfate and dichlorophenol indophenol cannot be reduced by the enzyme | Arabidopsis thaliana | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At4g20930 | - |
Arabidopsis thaliana |
| AtHDH1 | - |
Arabidopsis thaliana |
| HDH1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Arabidopsis thaliana |
| 40 | - |
the optimum activity is at about 40°C. Above 50°C, the enzyme activity declines | Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 55 | 20°C: about 90% of maximal activity, 50°C: about 60% of maximal activity | Arabidopsis thaliana |
| 20 | 45 | high activity between 20 and 45°C | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.652 | - |
NAD+ | 25°C, pH 8.5 | Arabidopsis thaliana | |
| 2.652 | - |
NAD+ | at pH 8.5 and 40°C | Arabidopsis thaliana | |
| 3.43 | - |
3-hydroxy-2-methylpropanoate | 25°C, pH 8.5 | Arabidopsis thaliana | |
| 3.43 | - |
(S)-3-hydroxyisobutyrate | at pH 8.5 and 40°C | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | pH 7.5: about 50% of maximal activity, pH 9.5: about 50% of maximal activity | Arabidopsis thaliana |
| 8 | 9 | high activity between pH 8.0 and 9.0 | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the enzyme is highly specific for NAD+ as a cofactor | Arabidopsis thaliana | |
| NAD+ | NADP+ is not a suitable cofactor | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | At4g20930 knockdown plants show increased root growth inhibition of the mutants if cultivated in the presence of Val or Ile but not in the presence of leucine | Arabidopsis thaliana |
| metabolism | the enzyme is involved in both, valine and isoleucine degradation in Arabidopsis thaliana | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.99 | - |
3-hydroxy-2-methylpropanoate | 25°C, pH 8.5 | Arabidopsis thaliana | |
| 4.993 | - |
(S)-3-hydroxyisobutyrate | at pH 8.5 and 25°C | Arabidopsis thaliana | |
| 7.57 | - |
NAD+ | 25°C, pH 8.5 | Arabidopsis thaliana | |
| 7.571 | - |
NAD+ | at pH 8.5 and 25°C | Arabidopsis thaliana | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
