Protein Variants | Comment | Organism |
---|---|---|
D33R | higher affinity to cofactor NADP+ than to natural cofactor NAD+ | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
NAD+ | wild type | Rattus norvegicus | |
0.056 | - |
L-3-hydroxyisobutyrate | wild type, NAD as cofactor | Rattus norvegicus | |
0.104 | - |
L-3-hydroxyisobutyrate | wild type, NADP as cofactor | Rattus norvegicus | |
0.11 | - |
L-3-hydroxyisobutyrate | mutant D33R, NADP as cofactor | Rattus norvegicus | |
0.112 | - |
L-3-hydroxyisobutyrate | mutant D33R, NAD as cofactor | Rattus norvegicus | |
0.24 | - |
NADP+ | mutant D33R | Rattus norvegicus | |
0.24 | - |
NADP+ | mutant enzyme D33R, pH and temperature not specified in the publication | Rattus norvegicus | |
0.96 | - |
NADP+ | wild type | Rattus norvegicus | |
0.96 | - |
NADP+ | wild type enzyme, pH and temperature not specified in the publication | Rattus norvegicus | |
1.24 | - |
NAD+ | mutant D33R | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-3-hydroxyisobutyrate + NAD+ | Rattus norvegicus | - |
L-2-methyl-3-oxopropionate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-beta-hydroxyisobutyrate + NADP+ | - |
Rattus norvegicus | 2-methyl-3-oxopropanoate + NADPH + H+ | - |
r | |
L-3-hydroxyisobutyrate + NAD+ | - |
Rattus norvegicus | L-2-methyl-3-oxopropionate + NADH | - |
? |