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Literature summary for 1.1.1.31 extracted from
- Purification, crystallization and some properties of beta-hydroxyisobutyrate dehydrogenase from candida rugosa IFO 0750 (1981), Agric. Biol. Chem., 45, 2805-2814.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| powdered ammonium sulfate is added to the enzyme solution until it becomes slightly turbid, after 4 days crystallization is complete | Diutina rugosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
NAD+ | - |
Diutina rugosa |  |
| 0.37 | - |
L-3-hydroxyisobutyrate | - |
Diutina rugosa | |
| 1.25 | - |
D-3-hydroxyisobutyrate | - |
Diutina rugosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
alpha2, 2 * 40000, SDS-PAGE | Diutina rugosa |
| 80000 | - |
gel filtration | Diutina rugosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Diutina rugosa | - |
IFO 0750 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Diutina rugosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-3-hydroxyisobutyrate + NAD+ | - |
Diutina rugosa | (S)-methylmalonate semialdehyde + NADH + H+ | - |
r | |
| L-3-hydroxyisobutyrate + NAD+ | - |
Diutina rugosa | (S)-methylmalonate semialdehyde + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, 2 * 40000, SDS-PAGE | Diutina rugosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Diutina rugosa | |
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