KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | Yamadazyma tenuis | |
0.5 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Yamadazyma tenuis | |
0.6 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
0.7 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
0.9 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
1.2 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
1.3 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
1.4 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
1.7 | - |
5,6-dideoxy-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia | |
2.4 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
2.5 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
2.6 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | Yamadazyma tenuis | |
4.5 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
4.7 | - |
oenanthaldehyde | pH 7, 25°C | [Candida] intermedia | |
5.4 | - |
oenanthaldehyde | pH 7, 25°C | Yamadazyma tenuis | |
8.9 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
14.7 | - |
5,6-dideoxy-6-fluoro-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia | |
50 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
64 | - |
D-xylose | pH 7, 25°C | Yamadazyma tenuis | |
74 | - |
D-galactose | pH 7, 25°C | [Candida] intermedia | |
228 | - |
D-galactose | pH 7, 25°C | Yamadazyma tenuis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | Yamadazyma tenuis | - |
xylitol + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yamadazyma tenuis | - |
- |
- |
[Candida] intermedia | A0A1L0DQY1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dideoxy-5,6-difluoro-D-glucofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5,6-dideoxy-5,6-difluoro-D-glucofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5,6-dideoxy-6-fluoro-D-xylohexofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
5,6-dideoxy-D-xylo-hexofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5,6-dideoxy-D-xylohexofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
5-azido-5-deoxy-D-xylofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5-azido-5-deoxy-D-xylofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
5-deoxy-D-xylofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5-deoxy-D-xylofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5-fluoro-5-deoxy-D-xylofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
5-fluoro-5-deoxy-D-xylofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
6-azido-5,6-dideoxy-D-xylo-hexofuranose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
6-azido-5,6-dideoxy-D-xylohexofuranose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
cyclohexanecarboxaldehyde + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
cyclohexanecarboxaldehyde + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
D-galactose + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
D-galactose + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? | |
D-xylose + NADH + H+ | - |
Yamadazyma tenuis | xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | - |
[Candida] intermedia | xylitol + NADP+ | - |
? | |
oenanthaldehyde + NADH + H+ | - |
Yamadazyma tenuis | ? + NAD+ | - |
? | |
oenanthaldehyde + NADH + H+ | - |
[Candida] intermedia | ? + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
oenanthaldehyde | pH 7, 25°C | [Candida] intermedia | |
5.9 | - |
D-galactose | pH 7, 25°C | [Candida] intermedia | |
10.5 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
12 | - |
D-galactose | pH 7, 25°C | Yamadazyma tenuis | |
12 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
13 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
13.5 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
14 | - |
D-xylose | pH 7, 25°C | Yamadazyma tenuis | |
14 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
14 | - |
5,6-dideoxy-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia | |
15.5 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
16 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
18 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
18 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
18 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
18 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | Yamadazyma tenuis | |
18 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Yamadazyma tenuis | |
19 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
20 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | Yamadazyma tenuis | |
21 | - |
oenanthaldehyde | pH 7, 25°C | Yamadazyma tenuis | |
21 | - |
5,6-dideoxy-6-fluoro-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme has dual coenzyme specificity, accepting both NADH and NADPH | Yamadazyma tenuis | |
NADH | enzyme has dual coenzyme specificity, accepting both NADH and NADPH | [Candida] intermedia | |
NADPH | enzyme has dual coenzyme specificity, accepting both NADH and NADPH | Yamadazyma tenuis | |
NADPH | enzyme has dual coenzyme specificity, accepting both NADH and NADPH | [Candida] intermedia |
General Information | Comment | Organism |
---|---|---|
metabolism | derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol | Yamadazyma tenuis |
metabolism | derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol | [Candida] intermedia |
physiological function | Candida intermedia produces two different isoforms. Isoform I is strictly specific for NADPH, isoform II shows similar specificity constants for NADPH and NADH | [Candida] intermedia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
D-galactose | pH 7, 25°C | Yamadazyma tenuis | |
0.08 | - |
D-galactose | pH 7, 25°C | [Candida] intermedia | |
0.15 | - |
6-azido-5,6-dideoxy-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia | |
0.21 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
0.22 | - |
D-xylose | pH 7, 25°C | Yamadazyma tenuis | |
0.35 | - |
5-azido-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
0.98 | - |
oenanthaldehyde | pH 7, 25°C | [Candida] intermedia | |
1.08 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | [Candida] intermedia | |
1.43 | - |
5,6-dideoxy-6-fluoro-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia | |
1.46 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
1.7 | - |
5-azido-5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
2.67 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
3.9 | - |
oenanthaldehyde | pH 7, 25°C | Yamadazyma tenuis | |
5.6 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
6.46 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
6.9 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | Yamadazyma tenuis | |
7.14 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | Yamadazyma tenuis | |
8.24 | - |
5,6-dideoxy-D-xylohexofuranose | pH 7, 25°C | [Candida] intermedia | |
10.38 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
12.86 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
15 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
20 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
22.86 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Yamadazyma tenuis | |
31.7 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | Yamadazyma tenuis | |
36 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Yamadazyma tenuis | |
297 | - |
NADH | pH 7, 25°C | [Candida] intermedia | |
548 | - |
NADPH | pH 7, 25°C | [Candida] intermedia |