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Literature summary for 1.1.1.307 extracted from

  • Guirimand, G.G.Y.; Bamba, T.; Matsuda, M.; Inokuma, K.; Morita, K.; Kitada, Y.; Kobayashi, Y.; Yukawa, T.; Sasaki, K.; Ogino, C.; Hasunuma, T.; Kondo, A.
    Combined cell surface display of beta-D-glucosidase (BGL), maltose transporter (MAL11), and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose coutilization for xylitol bioproduction from lignocellulosic B (2019), Biotechnol. J., 14, e1800704 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl1, recombinant expression of Xyl1 in Saccharomyces cerevisiae strain YPH499, co-expression with Aspergillus aculeatus beta-glucosidase 1 (AaBGL) and endogenous maltose transporter ScMAL11 Scheffersomyces stipitis

Protein Variants

Protein Variants Comment Organism
additional information xylitol is produced from lignocellulosic biomass by a recombinant strain of Saccharomyces cerevisiae strain YPH499-SsXR-AaBGL expressing cytosolic Scheffersomyces stipitis xylose reductase (SsXR), along with Aspergillus aculeatus beta-glucosidase 1 (AaBGL) displayed on the cell surface. The simultaneous co-fermentation of cellobiose/xylose by this strain leads to an about 2.5fold increase in xylitol/xylose ratio compared to the use of a glucose/xylose mixture as a substrate. Further improvement in the xylose uptake by the cell is achieved by a broad evaluation of several homologous and heterologous transporters. Homologous maltose transporter (ScMAL11) shows the best performance in xylose transport and is used to generate the strain YPH499-XRScMAL11-BGL with a significantly improved xylitol production capacity from cellobiose/xylose co-utilization. Method evaluation and optimization, overview Scheffersomyces stipitis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Scheffersomyces stipitis 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADPH + H+ Scheffersomyces stipitis
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Scheffersomyces stipitis NRRL Y-11545
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Scheffersomyces stipitis NBRC 10063
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Scheffersomyces stipitis ATCC 58785
-
xylitol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis P31867
-
-
Scheffersomyces stipitis ATCC 58785 P31867
-
-
Scheffersomyces stipitis NBRC 10063 P31867
-
-
Scheffersomyces stipitis NRRL Y-11545 P31867
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADPH + H+
-
Scheffersomyces stipitis xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Scheffersomyces stipitis NRRL Y-11545 xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Scheffersomyces stipitis NBRC 10063 xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Scheffersomyces stipitis ATCC 58785 xylitol + NADP+
-
r

Synonyms

Synonyms Comment Organism
SsXR
-
Scheffersomyces stipitis
XYL1
-
Scheffersomyces stipitis
xylose reductase
-
Scheffersomyces stipitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Scheffersomyces stipitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Scheffersomyces stipitis
NADPH
-
Scheffersomyces stipitis