Cloned (Comment) | Organism |
---|---|
gene xyl1, random multi-copy integration of the mutated KmXYL1 (mKmXYL1) gene is carried out using thermotolerant yeast Kluyveromyces marxianus strain KCTC17555DELTAURA3, in order to enhance xylitol production. After multi-copy integration, the highest xylitol producing strain is isolated and named Kluyveromyces marxianus 17555-JBP2. This strain exhibits 440% higher xylitol production than the parental strain at 30°C. Quantitative real-time PCR and transcriptome analysis demonstrate that, relative to the parent strain, Kluyveromyces marxianus strain 17555-JBP2 exhibits two more copies of mKmXY1 genes and a 9.63fold elevation in transcription of NAD(P)H-dependent XR | Kluyveromyces marxianus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the engineered Kluyveromyces marxianus strain 17555-JBP2 by random multi-copy integration of the mutated KmXYL1 gene exhibits two more copies of mKmXY1 genes and a 9.63fold elevation in transcription of NAD(P)H-dependent XR, optimization of bioreactor fermentation conditions (agitation speed), high-temperature (40°C) xylitol productivity of strain 17555-JBP2, which then shows an 81% improvement relative to the parental strain. Method development and optimization, overview | Kluyveromyces marxianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Kluyveromyces marxianus | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Kluyveromyces marxianus NBRC 104275 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Kluyveromyces marxianus BCC 29191 | - |
xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | Kluyveromyces marxianus DMKU3-1042 | - |
xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | W0T4K1 | - |
- |
Kluyveromyces marxianus BCC 29191 | W0T4K1 | - |
- |
Kluyveromyces marxianus DMKU3-1042 | W0T4K1 | - |
- |
Kluyveromyces marxianus NBRC 104275 | W0T4K1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | - |
Kluyveromyces marxianus | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Kluyveromyces marxianus NBRC 104275 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Kluyveromyces marxianus BCC 29191 | xylitol + NADP+ | - |
r | |
D-xylose + NADPH + H+ | - |
Kluyveromyces marxianus DMKU3-1042 | xylitol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
KmXYL1 | - |
Kluyveromyces marxianus |
NAD(P)H-dependent D-xylose reductase | UniProt | Kluyveromyces marxianus |
NAD(P)H-dependent XR | - |
Kluyveromyces marxianus |
xylose reductase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
higher activity compared to 30°C | Kluyveromyces marxianus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | assay at | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Kluyveromyces marxianus | |
NADPH | - |
Kluyveromyces marxianus |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of endogenous XR enhances xylitol productivity at 40°C by thermotolerant Kluyveromyces marxianus, high-temperature xylose consumption and xylitol production rates of the mKmXYL1 gene-overexpressing strain are compared to those of the parental strain KCTC17555DELTAURA3 | Kluyveromyces marxianus |