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Literature summary for 1.1.1.307 extracted from

  • Park, J.B.; Kim, J.S.; Kweon, D.H.; Kweon, D.H.; Seo, J.H.; Ha, S.J.
    Overexpression of endogenous xylose reductase enhanced xylitol productivity at 40°C by thermotolerant yeast Kluyveromyces marxianus (2019), Appl. Biochem. Biotechnol., 189, 459-470 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl1, random multi-copy integration of the mutated KmXYL1 (mKmXYL1) gene is carried out using thermotolerant yeast Kluyveromyces marxianus strain KCTC17555DELTAURA3, in order to enhance xylitol production. After multi-copy integration, the highest xylitol producing strain is isolated and named Kluyveromyces marxianus 17555-JBP2. This strain exhibits 440% higher xylitol production than the parental strain at 30°C. Quantitative real-time PCR and transcriptome analysis demonstrate that, relative to the parent strain, Kluyveromyces marxianus strain 17555-JBP2 exhibits two more copies of mKmXY1 genes and a 9.63fold elevation in transcription of NAD(P)H-dependent XR Kluyveromyces marxianus

Protein Variants

Protein Variants Comment Organism
additional information construction of the engineered Kluyveromyces marxianus strain 17555-JBP2 by random multi-copy integration of the mutated KmXYL1 gene exhibits two more copies of mKmXY1 genes and a 9.63fold elevation in transcription of NAD(P)H-dependent XR, optimization of bioreactor fermentation conditions (agitation speed), high-temperature (40°C) xylitol productivity of strain 17555-JBP2, which then shows an 81% improvement relative to the parental strain. Method development and optimization, overview Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADPH + H+ Kluyveromyces marxianus
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Kluyveromyces marxianus NBRC 104275
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Kluyveromyces marxianus BCC 29191
-
xylitol + NADP+
-
r
D-xylose + NADPH + H+ Kluyveromyces marxianus DMKU3-1042
-
xylitol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus W0T4K1
-
-
Kluyveromyces marxianus BCC 29191 W0T4K1
-
-
Kluyveromyces marxianus DMKU3-1042 W0T4K1
-
-
Kluyveromyces marxianus NBRC 104275 W0T4K1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADPH + H+
-
Kluyveromyces marxianus xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Kluyveromyces marxianus NBRC 104275 xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Kluyveromyces marxianus BCC 29191 xylitol + NADP+
-
r
D-xylose + NADPH + H+
-
Kluyveromyces marxianus DMKU3-1042 xylitol + NADP+
-
r

Synonyms

Synonyms Comment Organism
KmXYL1
-
Kluyveromyces marxianus
NAD(P)H-dependent D-xylose reductase UniProt Kluyveromyces marxianus
NAD(P)H-dependent XR
-
Kluyveromyces marxianus
xylose reductase
-
Kluyveromyces marxianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
higher activity compared to 30°C Kluyveromyces marxianus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 assay at Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Kluyveromyces marxianus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Kluyveromyces marxianus
NADPH
-
Kluyveromyces marxianus

General Information

General Information Comment Organism
malfunction overexpression of endogenous XR enhances xylitol productivity at 40°C by thermotolerant Kluyveromyces marxianus, high-temperature xylose consumption and xylitol production rates of the mKmXYL1 gene-overexpressing strain are compared to those of the parental strain KCTC17555DELTAURA3 Kluyveromyces marxianus