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Literature summary for 1.1.1.307 extracted from

  • Jeon, W.Y.; Yoon, B.H.; Ko, B.S.; Shim, W.Y.; Kim, J.H.
    Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis (2012), Bioprocess Biosyst. Eng., 35, 191-198.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.307

Protein Variants

Protein Variants Comment Organism
additional information xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene under control of a constitutive glyceraldehyde-3-phosphate dehydrogenase promoter in a Candida tropicalis xylitol dehydrogenase gene (XYL2)-disrupted strain resulting in recombinant strain LNG2, overview Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Candida tropicalis
-
 D-xylose + NADH + H+
-
 r
xylitol + NAD+ Candida tropicalis ATCC 20336
-
 D-xylose + NADH + H+
-
 r
xylitol + NADP+ Candida tropicalis
-
 D-xylose + NADPH + H+
-
 r
xylitol + NADP+ Candida tropicalis ATCC 20336
-
 D-xylose + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-
Candida tropicalis ATCC 20336
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.86
-
 recombinant strain LNG2, pH 7.0, 25°C, xylose reduction with NADPH Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
 Candida tropicalis D-xylose + NADH + H+
-
 r
xylitol + NAD+
-
 Candida tropicalis ATCC 20336 D-xylose + NADH + H+
-
 r
xylitol + NADP+
-
 Candida tropicalis D-xylose + NADPH + H+
-
 r
xylitol + NADP+
-
 Candida tropicalis ATCC 20336 D-xylose + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
xylose reductase
-
 Candida tropicalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Candida tropicalis
NADH
-
 Candida tropicalis
NADP+
-
 Candida tropicalis
NADPH
-
 Candida tropicalis

Expression

Organism Comment Expression
Candida tropicalis formation of xylose reductase in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose down

General Information

General Information Comment Organism
metabolism xylose reductase is the first enzyme in D-xylose metabolism, catalyzing the reduction of D-xylose to xylitol Candida tropicalis