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Literature summary for 1.1.1.306 extracted from
- NAD-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from Amycolatopsis methanolica (1992), Eur. J. Biochem., 206, 511-518.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| factor | unidentified factor that is required for maximal activity with formaldehyde as substrate, only 4% activity in its absence, oxidation of alcohols do not require the factor | Amycolatopsis methanolica | |
| methanol | can replace an unidentified activating factor | Amycolatopsis methanolica |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 80% inhibition at 5 mM | Amycolatopsis methanolica | |
| 2-(cyclohexylamino)ethanesulfonic acid-NaOH | 25 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
| acetaldehyde | 15% inhibition at 1 mM | Amycolatopsis methanolica | |
| Cu2+ | complete inhibition at 1 mM | Amycolatopsis methanolica | |
| glycine-NaOH | 100 mM at pH 9.0: 40% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
| Hg2+ | complete inhibition at 0.1 mM | Amycolatopsis methanolica | |
| KCN | 55% inhibition at 2 mM | Amycolatopsis methanolica | |
| Na2B4O7-HCl | 100 mM at pH 9.0: no activity | Amycolatopsis methanolica | |
| Tris-HCl | 50 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0 | Amycolatopsis methanolica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
1-Octanol | - |
Amycolatopsis methanolica | |
| 1.7 | - |
12-Hydroxydodecanoic acid | - |
Amycolatopsis methanolica | |
| 3.4 | - |
1-Hexanol | - |
Amycolatopsis methanolica | |
| 9.6 | - |
formaldehyde | - |
Amycolatopsis methanolica | |
| 26.5 | - |
1-Pentanol | - |
Amycolatopsis methanolica | |
| 84 | - |
1-butanol | - |
Amycolatopsis methanolica | |
| 324 | - |
1-propanol | - |
Amycolatopsis methanolica | |
| 343 | - |
ethanol | - |
Amycolatopsis methanolica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 6 atoms Zn/enzyme molecule | Amycolatopsis methanolica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
| 120000 | - |
gel filtration | Amycolatopsis methanolica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ | Amycolatopsis methanolica | - |
S-formylmycothiol + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Amycolatopsis methanolica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Amycolatopsis methanolica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | induction of enzyme and factor synthesis only if cells grown in methanol-containing medium | Amycolatopsis methanolica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.03 | - |
cell free extract, in the absence of an unknown activating factor | Amycolatopsis methanolica |
| 0.08 | - |
cell free extract, in the presence of an unknown activating factor | Amycolatopsis methanolica |
| 6.9 | - |
substrate: ethanol | Amycolatopsis methanolica |
| 15 | - |
substrate: formaldehyde | Amycolatopsis methanolica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-butanol + NAD+ | - |
Amycolatopsis methanolica | n-butanal + NADH | - |
? | |
| 1-hexanol + NAD+ | - |
Amycolatopsis methanolica | hexanal + NADH + H+ | - |
r | |
| 1-octanol + NAD+ | - |
Amycolatopsis methanolica | octanal + NADH + H+ | - |
r | |
| 1-pentanol + NAD+ | - |
Amycolatopsis methanolica | pentanal + NADH + H+ | - |
r | |
| 1-propanol + NAD+ | - |
Amycolatopsis methanolica | propanal + NADH + H+ | - |
r | |
| 12-hydroxydodecanoic acid + NADH | - |
Amycolatopsis methanolica | ? + NAD+ | - |
? | |
| ethanol + NAD+ | - |
Amycolatopsis methanolica | ethanal + NADH + H+ | - |
r | |
| formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+ | - |
Amycolatopsis methanolica | S-formylmycothiol + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 40000, SDS-PAGE | Amycolatopsis methanolica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FD-FA1DH | - |
Amycolatopsis methanolica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
formaldehyde as substrate | Amycolatopsis methanolica |
| 10.2 | - |
alcohols as substrates | Amycolatopsis methanolica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Amycolatopsis methanolica | |
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