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Literature summary for 1.1.1.304 extracted from
- Properties of diacetyl (acetoin) reductase from Bacillus stearothermophilus (1996), Bioorg. Med. Chem., 4, 1197-1201.
General Stability
| General Stability | Organism |
|---|---|
| unstable to dilution, kept diluted at 0°C for ca. 60 min it will lose 62% of activity. This inactivation is almost completely reversed by the addition of NAD+ | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19 | - |
diacetyl | pH 7.5, 25°C | Geobacillus stearothermophilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
2 * 26000, SDS-PAGE | Geobacillus stearothermophilus |
| 49000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71.4 | - |
pH 7.5, 25°C | Geobacillus stearothermophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| storage at 0°C in the presence of 20% glycerol, 0.1 mM EDTA, 5 mM 2-mercaptoethanol and 0.6 mM NAD+ in TEA buffer, pH 7.5, half-life of one month | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacetyl + NADH + H+ | - |
Geobacillus stearothermophilus | (S)-acetoin + NAD+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Geobacillus stearothermophilus | |
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Release 2023.1 (January 2023)
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