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Literature summary for 1.1.1.303 extracted from

  • Tang, W.; Lian, C.; Si, Y.; Chang, J.
    Purification and characterization of (2R,3R)-2,3-butanediol dehydrogenase of the human pathogen Neisseria gonorrhoeae FA1090 produced in Escherichia coli (2021), Mol. Biotechnol., 63, 491-501.
    View publication on PubMed
Search for more literature for 1.1.1.303

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diacetyl + NADH + H+ Neisseria gonorrhoeae
-
 (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+ Neisseria gonorrhoeae FA1090
-
 (R)-acetoin + NAD+
-
 ir

Organism

Organism UniProt Comment Textmining
Neisseria gonorrhoeae
-
-
-
Neisseria gonorrhoeae FA1090
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl + NADH + H+
-
 Neisseria gonorrhoeae (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+ 60% activity compared to main activity of 2,3-butanediol dehydrogenase, i.e. oxidation of (2R,3R)-2,3-butanediol and reduction of (R)-acetoin, EC 1.1.1.4 Neisseria gonorrhoeae (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+
-
 Neisseria gonorrhoeae FA1090 (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+ 60% activity compared to main activity of 2,3-butanediol dehydrogenase, i.e. oxidation of (2R,3R)-2,3-butanediol and reduction of (R)-acetoin, EC 1.1.1.4 Neisseria gonorrhoeae FA1090 (R)-acetoin + NAD+
-
 ir

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Neisseria gonorrhoeae

Synonyms

Synonyms Comment Organism
acetoin/diacetyl reductase
-
 Neisseria gonorrhoeae
gutB
-
 Neisseria gonorrhoeae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Neisseria gonorrhoeae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Neisseria gonorrhoeae

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Neisseria gonorrhoeae

General Information

General Information Comment Organism
physiological function the enzyme is a 2,3-butanediol dehydrogenase (BDH, EC 1.1.1.4) and also a acetoin/diacetyl reductase, that catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol and the reverse reduction of (R)-acetoin to (2R,3R)-2,3-butanediol, as well as the reduction of diacetyl to (R)-acetoin. It is a pivotal enzyme for the formation of 2,3-butanediol (2,3-BD), a chiral compound with potential roles in the virulence of certain pathogens Neisseria gonorrhoeae