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Literature summary for 1.1.1.302 extracted from
- 2,5-Diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases of fungi and archaea (2008), FEBS J., 275, 4403-4414.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Aquifex aeolicus |
| expression in Escherichia coli | [Candida] glabrata |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25300 | - |
2 * 25300, SDS-PAGE | Aquifex aeolicus |
| 28500 | - |
2 * 28500, SDS-PAGE | [Candida] glabrata |
| 50600 | - |
sedimentation equilibrium analysis | Aquifex aeolicus |
| 57800 | - |
sedimentation equilibrium analysis | [Candida] glabrata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Methanocaldococcus jannaschii | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? |  |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Aquifex aeolicus | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | [Candida] glabrata | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Methanocaldococcus jannaschii DSM 2661 | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | [Candida] glabrata ATCC 2001 | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O66747 | - |
- |
| Methanocaldococcus jannaschii | Q58085 | - |
- |
| Methanocaldococcus jannaschii DSM 2661 | Q58085 | - |
- |
| [Candida] glabrata | Q6FU96 | - |
- |
| [Candida] glabrata ATCC 2001 | Q6FU96 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aquifex aeolicus |
- |
[Candida] glabrata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Methanocaldococcus jannaschii | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Aquifex aeolicus | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | [Candida] glabrata | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation | Aquifex aeolicus | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | Methanocaldococcus jannaschii | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | [Candida] glabrata | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Methanocaldococcus jannaschii DSM 2661 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | Methanocaldococcus jannaschii DSM 2661 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | [Candida] glabrata ATCC 2001 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
| 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | [Candida] glabrata ATCC 2001 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 28500, SDS-PAGE | [Candida] glabrata |
| homodimer | 2 * 25300, SDS-PAGE | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
Methanocaldococcus jannaschii |
| 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
Aquifex aeolicus |
| 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
[Candida] glabrata |
| arfC | - |
Methanocaldococcus jannaschii |
| MJ0671 | locus name | Methanocaldococcus jannaschii |
| ribD2 | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanocaldococcus jannaschii |
| 37 | - |
assay at | Aquifex aeolicus |
| 37 | - |
assay at | [Candida] glabrata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Methanocaldococcus jannaschii |
| 8.2 | - |
assay at | [Candida] glabrata |
| 8.2 | - |
asay at | Aquifex aeolicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | biosynthesis of riboflavin | Methanocaldococcus jannaschii |
| physiological function | biosynthesis of riboflavin | Aquifex aeolicus |
| physiological function | biosynthesis of riboflavin | [Candida] glabrata |
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