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Literature summary for 1.1.1.302 extracted from

  • Römisch-Margl, W.; Eisenreich, W.; Haase, I.; Bacher, A.; Fischer, M.
    2,5-Diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases of fungi and archaea (2008), FEBS J., 275, 4403-4414.
    View publication on PubMed
Search for more literature for 1.1.1.302

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus
expression in Escherichia coli Nakaseomyces glabratus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25300
-
 2 * 25300, SDS-PAGE Aquifex aeolicus
28500
-
 2 * 28500, SDS-PAGE Nakaseomyces glabratus
50600
-
 sedimentation equilibrium analysis Aquifex aeolicus
57800
-
 sedimentation equilibrium analysis Nakaseomyces glabratus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Methanocaldococcus jannaschii biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Aquifex aeolicus biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Nakaseomyces glabratus biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Methanocaldococcus jannaschii DSM 2661 biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Nakaseomyces glabratus ATCC 2001 biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O66747
-
-
Methanocaldococcus jannaschii Q58085
-
-
Methanocaldococcus jannaschii DSM 2661 Q58085
-
-
Nakaseomyces glabratus Q6FU96
-
-
Nakaseomyces glabratus ATCC 2001 Q6FU96
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aquifex aeolicus
-
 Nakaseomyces glabratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Methanocaldococcus jannaschii 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Aquifex aeolicus 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Nakaseomyces glabratus 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate Methanocaldococcus jannaschii 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation Aquifex aeolicus 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate Nakaseomyces glabratus 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Methanocaldococcus jannaschii DSM 2661 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate Methanocaldococcus jannaschii DSM 2661 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Nakaseomyces glabratus ATCC 2001 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate Nakaseomyces glabratus ATCC 2001 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 25300, SDS-PAGE Aquifex aeolicus
homodimer 2 * 28500, SDS-PAGE Nakaseomyces glabratus

Synonyms

Synonyms Comment Organism
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
-
 Methanocaldococcus jannaschii
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
-
 Aquifex aeolicus
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
-
 Nakaseomyces glabratus
arfC
-
 Methanocaldococcus jannaschii
MJ0671 locus name Methanocaldococcus jannaschii
ribD2
-
 Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aquifex aeolicus
37
-
 assay at Methanocaldococcus jannaschii
37
-
 assay at Nakaseomyces glabratus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Methanocaldococcus jannaschii
8.2
-
 asay at Aquifex aeolicus
8.2
-
 assay at Nakaseomyces glabratus

General Information

General Information Comment Organism
physiological function biosynthesis of riboflavin Methanocaldococcus jannaschii
physiological function biosynthesis of riboflavin Aquifex aeolicus
physiological function biosynthesis of riboflavin Nakaseomyces glabratus